Rabbit anti-Human PSTPIP1 Polyclonal Antibody | anti-PSTPIP1 antibody

PSTPIP1, NT (PSTPIP1, CD2BP1, Proline-serine-threonine phosphatase-interacting protein 1, CD2-binding protein 1, H-PIP) (Azide free) (HRP)

Cat. Num. AAA6338396

• Gene Names: PSTPIP1; H-PIP; PAPAS; CD2BP1; PSTPIP; CD2BP1L; CD2BP1S
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PSTPIP1; Polyclonal Antibody; NT (PSTPIP1; CD2BP1; Proline-serine-threonine phosphatase-interacting protein 1; CD2-binding protein 1; H-PIP) (Azide free) (HRP); H-PIP; anti-PSTPIP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PSTPIP1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PSTPIP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 89-118 in the N-terminal region of human PSTPIP1. Species sequence homology: mouse
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS648588. This demonstrates that MBS648588 detected the PSTPIP1 protein (arrow).)

Testing Data

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Related Product Information for anti-PSTPIP1 antibody

Proline-serine-threonine Phosphatase-interacting Protein 1 (PSTPIP1) binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, negatively affecting CD2-triggered T cell activation. PSTPIP1 appears to be a scaffold protein and a regulator of the actin cytoskeleton. It has also been shown to bind ABL1, PTPN18, WAS, CD2AP, and PTPN12. Mutations in the gene encoding this protein are a cause of PAPA syndrome.

Product Categories/Family for anti-PSTPIP1 antibody

Antibodies; CD Markers

References

1. Andre, M.F., et al., Dig. Dis. Sci. 55(6): 1681-1688 (2010). 2. Rose, J., et al., Mol. Med. 16(7-8): 247-253 (2010). 3. Hong, J.B., et al., J. Am. Acad. Dermatol. 61(3): 533-535 (2009). 4. Voss, M., et al., BMC Immunol. 10: 53 (2009). 5. Waite, A.L., et al., PLoS ONE 4(7): E6147 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 20149528
• NCBI GeneID: 9051
• NCBI Accession #: NP_003969.2
• NCBI GenBank Nucleotide #: NM_003978.3
• UniProt Accession #: O43586; O43585; O95657; B5BU74; B5BUK4
• Molecular Weight: 45,354 Da
• NCBI Official Full Name: proline-serine-threonine phosphatase-interacting protein 1
• NCBI Official Synonym Full Names: proline-serine-threonine phosphatase interacting protein 1
• NCBI Official Symbol: PSTPIP1
• NCBI Official Synonym Symbols: H-PIP; PAPAS; CD2BP1; PSTPIP; CD2BP1L; CD2BP1S
• NCBI Protein Information: proline-serine-threonine phosphatase-interacting protein 1; CD2 antigen-binding protein 1; CD2 cytoplasmic tail-binding protein; CD2-binding protein 1; PEST phosphatase-interacting protein 1
• UniProt Protein Name: Proline-serine-threonine phosphatase-interacting protein 1
• Protein Family: Proline-serine-threonine phosphatase-interacting protein
• UniProt Gene Name: PSTPIP1
• UniProt Synonym Gene Names: CD2BP1; PEST phosphatase-interacting protein 1
• UniProt Entry Name: PPIP1_HUMAN

NCBI Description

The protein encoded by this gene binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, negatively affecting CD2-triggered T cell activation. The encoded protein appears to be a scaffold protein and a regulator of the actin cytoskeleton. It has also been shown to bind ABL1, PTPN18, WAS, CD2AP, and PTPN12. Mutations in this gene are a cause of PAPA syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

PSTPIP1: an SH3-domain containing protein that may play a role in vesicle formation and transport. Interacts with PTP-PEST to inhibit WASp-driven actin polymerization and synapse formation.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 15q24.3

Cellular Component: membrane; lamellipodium; perinuclear region of cytoplasm; contractile ring; stress fiber; uropod; cytosol; cleavage furrow

Molecular Function: protein binding; actin binding; protein phosphatase binding

Biological Process: innate immune response; endocytosis; inflammatory response; signal transduction; cell adhesion

Disease: Pyogenic Sterile Arthritis, Pyoderma Gangrenosum, And Acne