Human STAT3 (Signal Transducer and Activator of Transcription 3) CLIA Kit | STAT3 clia kit

Human STAT3 (Signal Transducer and Activator of Transcription 3) CLIA Kit

Cat. Num. AAA2532069

• Reactivity: Human
• Synonyms: STAT3 (Signal Transducer and Activator of Transcription 3); Human STAT3 (Signal Transducer and Activator of Transcription 3) CLIA Kit; STAT3 clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant STAT3. No significant cross-reactivity or interference between STAT3 and analogues was observed.
• Sequence Length: 770

• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STAT3 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of STAT3 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to STAT3. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for STAT3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain STAT3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of STAT3. You can calculate the concentration of STAT3 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6981592
• NCBI GeneID: 25125
• NCBI Accession #: NP_036879.1
• NCBI GenBank Nucleotide #: NM_012747.2
• Molecular Weight: 88,040 Da
• NCBI Official Full Name: signal transducer and activator of transcription 3
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 3 (acute-phase response factor)
• NCBI Official Symbol: Stat3
• NCBI Protein Information: signal transducer and activator of transcription 3
• UniProt Protein Name: Signal transducer and activator of transcription 3
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: Stat3
• UniProt Entry Name: STAT3_RAT

NCBI Description

transcription factor that plays a role in induction of gene expression during acute phase response [RGD, Feb 2006]

Uniprot Description

STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; DNA-binding; Nuclear receptor co-regulator; Transcription factor

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nuclear chromatin; mitochondrial inner membrane; plasma membrane; nucleus; cytosol

Molecular Function: protein dimerization activity; protein binding; signal transducer activity; ligand-dependent nuclear receptor activity; DNA binding; sequence-specific DNA binding; transcription factor binding; protein phosphatase binding; protein kinase binding; transcription factor activity; CCR5 chemokine receptor binding; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; response to peptide hormone stimulus; positive regulation of transcription, DNA-dependent; radial glial cell differentiation; thermoregulation; glucose homeostasis; response to organic cyclic substance; response to estradiol stimulus; response to organic substance; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; astrocyte differentiation; protein import into nucleus; acute-phase response; negative regulation of glycolysis; positive regulation of Notch signaling pathway; aging; response to drug; intracellular receptor-mediated signaling pathway; eating behavior; stem cell maintenance; regulation of multicellular organism growth; JAK-STAT cascade; cellular response to hormone stimulus; regulation of transcription from RNA polymerase II promoter; cell proliferation; response to ethanol; sexual reproduction; response to cytokine stimulus; positive regulation of transcription from RNA polymerase II promoter; regulation of mitochondrial membrane permeability; eye photoreceptor cell differentiation; phosphorylation; negative regulation of apoptosis