Human PPP1CC (Protein phosphatase 1C catalytic subunit) ELISA Kit | PPP1CC elisa kit

Human PPP1CC (Protein phosphatase 1C catalytic subunit) ELISA Kit

Cat. Num. AAA7612174

• Gene Names: PPP1CC; PP1C; PP-1G; PPP1G
• Reactivity: Human
• Synonyms: PPP1CC (Protein phosphatase 1C catalytic subunit); Human PPP1CC (Protein phosphatase 1C catalytic subunit) ELISA Kit; PPP1CC; Protein phosphatase 1C catalytic subunit; PP-1G; Serine/threonine-protein phosphatase PP1-gamma catalytic subunit; PPP1CC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PPP1CC. No significant cross-reactivity or interference between PPP1CC and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PPP1CC elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 548573
• NCBI GeneID: 5501
• UniProt Accession #: P36873
• Molecular Weight: 323
• NCBI Official Full Name: Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
• NCBI Official Synonym Full Names: protein phosphatase 1, catalytic subunit, gamma isozyme
• NCBI Official Symbol: PPP1CC
• NCBI Official Synonym Symbols: PP1C; PP-1G; PPP1G
• NCBI Protein Information: serine/threonine-protein phosphatase PP1-gamma catalytic subunit; serine/threonine phosphatase 1 gamma; protein phosphatase 1C catalytic subunit; protein phosphatase 1, catalytic subunit, gamma isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP1CC
• UniProt Synonym Gene Names: PP-1G
• UniProt Entry Name: PP1G_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

PPP1CC: is a catalytic subunit of protein phosphatase 1 (PP1), a ubiquitous cytosolic serine-threonine phosphatase. Composed a catalytic subunit (PPP1CA, PPP1CB or PPP1CC) which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting (or regulatory) subunits: PPP1R12A and PPP1R12B mediate binding to myosin; PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen; and PPP1R15A and PPP1R15B mediate binding to EIF2S1. PP1 is essential for cell division, plays critical roles in many cellular processes including glycogen metabolism, muscle contractility and protein synthesis, and is involved in the regulation of ionic conductances and long-term synaptic plasticity. Inhibits the synthesis of proteins involved in synaptic plasticity. Binds to the transcription factor cyclic AMP-dependent response element binding (CREB) and dephosphorylates it. May regulate chromatin condensation through regulation of histone H3 phosphorylation. Differentially expressed in gastric cancer. May participate in the neurodegenerative progress in Alzheimer's disease. Down-regulated in lung squamous cell carcinoma.

Protein type: EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor); Nucleolus

Chromosomal Location of Human Ortholog: 12q24.1-q24.2

Cellular Component: focal adhesion; protein complex; mitochondrion; cytoplasm; nucleolus; nuclear speck; midbody; cytosol; nucleus; cleavage furrow

Molecular Function: protein binding; metal ion binding; phosphoric monoester hydrolase activity; protein serine/threonine phosphatase activity; phosphoprotein phosphatase activity; protein kinase binding

Biological Process: glycogen metabolic process; cell division; transforming growth factor beta receptor signaling pathway; triacylglycerol catabolic process; mitotic cell cycle; circadian regulation of gene expression; regulation of circadian rhythm; protein amino acid dephosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; entrainment of circadian clock by photoperiod