Rat Signal transducer and activator of transcription 3 ELISA Kit | Stat3 elisa kit

Rat Signal transducer and activator of transcription 3 ELISA Kit

Cat. Num. AAA760293

• Reactivity: Rat
• Synonyms: Signal transducer and activator of transcription 3; Rat Signal transducer and activator of transcription 3 ELISA Kit; Stat3/Signal transducer and activator of transcription 3; Stat3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of SRX1. No significant cross-reactivity or interference between SRX1 and analogues was observed.
• Sequence Length: 770

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SRX1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SRX1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Stat3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-SRX1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-SRX1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SRX1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SRX1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 6981592
• NCBI GeneID: 25125
• NCBI Accession #: NP_036879.1
• NCBI GenBank Nucleotide #: NM_012747.2
• Molecular Weight: 88,040 Da
• NCBI Official Full Name: signal transducer and activator of transcription 3
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 3 (acute-phase response factor)
• NCBI Official Symbol: Stat3
• NCBI Protein Information: signal transducer and activator of transcription 3
• UniProt Protein Name: Signal transducer and activator of transcription 3
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: Stat3
• UniProt Entry Name: STAT3_RAT

NCBI Description

transcription factor that plays a role in induction of gene expression during acute phase response [RGD, Feb 2006]

Uniprot Description

STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; DNA-binding

Cellular Component: cytoplasm; cytosol; mitochondrial inner membrane; mitochondrion; nuclear chromatin; nucleoplasm; nucleus; plasma membrane

Molecular Function: CCR5 chemokine receptor binding; chromatin DNA binding; DNA binding; glucocorticoid receptor binding; identical protein binding; ligand-dependent nuclear receptor activity; protein binding; protein dimerization activity; protein kinase binding; protein phosphatase binding; sequence-specific DNA binding; signal transducer activity; transcription factor activity; transcription factor binding

Biological Process: acute-phase response; aging; astrocyte differentiation; cell proliferation; cellular response to hormone stimulus; eating behavior; eye photoreceptor cell differentiation; glucose homeostasis; intracellular receptor-mediated signaling pathway; JAK-STAT cascade; leptin-mediated signaling pathway; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of glycolysis; phosphorylation; positive regulation of cell proliferation; positive regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein import into nucleus; radial glial cell differentiation; regulation of cell cycle; regulation of mitochondrial membrane permeability; regulation of multicellular organism growth; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to cytokine stimulus; response to drug; response to estradiol stimulus; response to ethanol; response to organic cyclic substance; response to organic substance; response to peptide hormone stimulus; sexual reproduction; stem cell maintenance; thermoregulation; transcription from RNA polymerase II promoter