Rabbit anti-Human, Mouse B3GNT6 Polyclonal Antibody | anti-B3GNT6 antibody

B3GNT6, ID (B3GNT6, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6, Core 3 synthase) (FITC)

Cat. Num. AAA6277165

• Gene Names: B3GNT6; BGnT-6; B3Gn-T6; beta3Gn-T6; beta-1,3-Gn-T6
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: B3GNT6; Polyclonal Antibody; ID (B3GNT6; UDP-GlcNAc:betaGal beta-1; 3-N-acetylglucosaminyltransferase 6; Core 3 synthase) (FITC); Core 3 synthase; anti-B3GNT6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-B3GNT6 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: B3GNT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152~182 amino acids from the Central region of human B3GNT6.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of B3GNT6 Antibody (Center) in mouse brain tissue lysates (35ug/lane). B3GNT6 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(B3GNT6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the B3GNT6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-B3GNT6 antibody

Antibodies; Enzymes; Glycosyltransferase

References

Lee, S.H., et al. J. Biol. Chem. 284(25):17157-17169(2009). Iwai, T., et al. Proc. Natl. Acad. Sci. U.S.A. 102(12):4572-4577(2005). Iwai, T., et al. J. Biol. Chem. 277(15):12802-12809(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 19263732
• NCBI GeneID: 192134
• UniProt Accession #: Q6ZMB0; Q6ZSC5; Q8TAZ4; Q8TDX1; E9PKS1
• Molecular Weight: 42,748 Da
• NCBI Official Full Name: B3GNT6 protein
• NCBI Official Synonym Full Names: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 (core 3 synthase)
• NCBI Official Symbol: B3GNT6
• NCBI Official Synonym Symbols: BGnT-6; B3Gn-T6; beta3Gn-T6; beta-1,3-Gn-T6
• NCBI Protein Information: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6; core 3 synthase; beta-1,3-N-acetylglucosaminyltransferase protein
• UniProt Protein Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
• Protein Family: Acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase
• UniProt Gene Name: B3GNT6
• UniProt Synonym Gene Names: BGnT-6; Beta-1,3-Gn-T6; Beta-1,3-N-acetylglucosaminyltransferase 6; Beta3Gn-T6
• UniProt Entry Name: B3GN6_HUMAN

Uniprot Description

B3GNT6: Beta-1,3-N-acetylglucosaminyltransferase that synthesizes the core 3 structure of the O-glycan, an important precursor in the biosynthesis of mucin-type glycoproteins. Plays an important role in the synthesis of mucin-type O-glycans in digestive organs. Belongs to the glycosyltransferase 31 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Membrane protein, integral; Glycan Metabolism - O-glycan biosynthesis; EC 2.4.1.-

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity; galactosyltransferase activity

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; glycoprotein biosynthetic process; O-glycan processing; O-glycan processing, core 3; post-translational protein modification