Human tauPK1 (Tau-Protein Kinase) CLIA Kit | tPK1 clia kit

Human tauPK1 (Tau-Protein Kinase) CLIA Kit

Cat. Num. AAA2530911

• Reactivity: Human
• Synonyms: tauPK1 (Tau-Protein Kinase); Human tauPK1 (Tau-Protein Kinase) CLIA Kit; Human tPK1 (Tau-Protein Kinase) CLIA Kit; Human tPK1; tPK1 clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant tauPK1. No significant cross-reactivity or interference between tauPK1 and analogues was observed.
• Sequence Length: 420

• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for tPK1 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of tauPK1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to tauPK1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for tauPK1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain tauPK1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of tauPK1. You can calculate the concentration of tauPK1 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 402652
• NCBI GeneID: 84027
• UniProt Accession #: P18266
• Molecular Weight: 46,742 Da
• NCBI Official Full Name: tau-protein kinase
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: Gsk3b
• NCBI Protein Information: glycogen synthase kinase-3 beta
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• Protein Family: Thiamin pyrophosphokinase
• UniProt Gene Name: Gsk3b
• UniProt Synonym Gene Names: GSK-3 beta; FA
• UniProt Entry Name: GSK3B_RAT

NCBI Description

mediates Par6-atypical protein kinase C (aPKC) complex regulation of cell polarity; may induce apoptosis [RGD, Feb 2006]

Uniprot Description

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation

Protein type: EC 2.7.11.26; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Kinase, protein; CMGC group; GSK family; GSK subfamily

Cellular Component: centrosome; protein complex; mitochondrion; dendrite; dendritic spine; cytosol; ribonucleoprotein complex; beta-catenin destruction complex; lipid raft; nucleoplasm; growth cone; cell soma; membrane; perinuclear region of cytoplasm; axon; cytoplasm; plasma membrane; dendritic shaft; nucleus

Molecular Function: p53 binding; beta-catenin binding; transcription factor binding; protein kinase binding; protein kinase activity; integrin binding; protein serine/threonine kinase activity; NF-kappaB binding; ionotropic glutamate receptor binding; protein binding; ubiquitin protein ligase binding; tau-protein kinase activity; kinase activity; tau protein binding; ATP binding

Biological Process: fat cell differentiation; circadian rhythm; glycogen metabolic process; negative regulation of MAP kinase activity; re-entry into mitotic cell cycle; genetic imprinting; positive regulation of apoptosis; positive regulation of axon extension; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; negative regulation of protein binding; ER overload response; response to drug; regulation of microtubule-based process; negative regulation of nitric-oxide synthase activity; myotube differentiation; negative regulation of dendrite morphogenesis; regulation of axon extension; negative regulation of NFAT protein import into nucleus; negative regulation of neuron maturation; organ morphogenesis; positive regulation of protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; response to activity; negative regulation of apoptosis; regulation of neuronal synaptic plasticity; positive regulation of protein binding; regulation of dendrite morphogenesis; positive regulation of smooth muscle cell proliferation; protein amino acid autophosphorylation; response to lithium ion; establishment and/or maintenance of cell polarity; regulation of axonogenesis; response to estradiol stimulus; response to insulin stimulus; positive regulation of protein export from nucleus; positive regulation of neuron apoptosis; protein export from nucleus; positive regulation of cell-matrix adhesion; myoblast fusion; aging; cell migration; Wnt receptor signaling pathway; hippocampus development; positive regulation of peptidyl-serine phosphorylation; peptidyl-serine phosphorylation; positive regulation of protein complex assembly; axonogenesis; establishment of cell polarity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; epithelial to mesenchymal transition; negative regulation of protein complex assembly; phosphorylation; positive regulation of GTPase activity