Rabbit anti-Human, Mouse PIWI-L2 Polyclonal Antibody | anti-PIWIL2 antibody

PIWI-L2 Antibody

Cat. Num. AAA150536

• Gene Names: PIWIL2; CT80; HILI; mili; PIWIL1L
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: PIWI-L2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: PIWI-L2; Polyclonal Antibody; PIWI-L2 Antibody; CT80; HILI; mili; PIWIL1L; Piwi-like protein 2; piwi-like 2; anti-PIWIL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least three isoforms of PIWI-L2 are known to exist; this antibody will only detect the two longest isoforms. PIWI-L2 antibody is predicted to not cross-react with other PIWI protein family members.
• Purity/Purification: PIWI-L2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; PIWI-L2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 973

• Applicable Applications for anti-PIWIL2 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: PIWI-L2 antibody can be used for detection of PIWI-L2 by Western blot at 1 mug/mL.; Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples.; All other applications and species not yet tested
• Conjugate: Unconjugated
• Immunogen: PIWI-L2 antibody was raised against a 17 amino acid synthetic peptide near the center of human PIWI-L2. The immunogen is located within amino acids 410-460 of PIWI-L2
• Preparation and Storage: PIWI-L2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PIWI-L2 in HepG2 cell lysate with PIWI-L1 antibody at 1 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of PIWI-L2 in HepG2 cells with PIWI-L2 antibody at 2.5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of PIWI-L2 in HepG2 cells with PIWI-L2 antibody at 5 μg/ml.)

Related Product Information for anti-PIWIL2 antibody

PIWI-L2 Antibody: PIWI-L2 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L2 has been implicated in multiple roles including stem cell self-renewal and is highly expressed in many breast cancers. In these tumors, PIWI-L2 silences the expression of STAT3, a regulator of Bcl-XL and cyclin D1, allowing the proliferation and survival of breast cancer stem cells. Recent studies have shown that overexpression of PIWI-like proteins are potential biomarkers for colon and other cancers.

NCBI and Uniprot Product Information

• NCBI GI #: 24431985
• NCBI GeneID: 55124
• NCBI Accession #: NP_00129193
• NCBI GenBank Nucleotide #: NM_018068.3
• UniProt Accession #: Q8TC59; Q96SW6; Q9NW28; A8K4S3; A8K8S5; B0AZN9; B0AZP2; B4DR22; E7ECA4
• Molecular Weight: 105,760 Da
• NCBI Official Full Name: piwi-like protein 2
• NCBI Official Synonym Full Names: piwi-like RNA-mediated gene silencing 2
• NCBI Official Symbol: PIWIL2
• NCBI Official Synonym Symbols: CT80; HILI; mili; PIWIL1L
• NCBI Protein Information: piwi-like protein 2; Miwi like; piwi-like 2; piwil2-like protein; cancer/testis antigen 80; 80kDa PIWIL2 short isoform
• UniProt Protein Name: Piwi-like protein 2
• Protein Family: Piwi-like protein
• UniProt Gene Name: PIWIL2
• UniProt Synonym Gene Names: HILI; CT80
• UniProt Entry Name: PIWL2_HUMAN

NCBI Description

PIWIL2 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008]

Uniprot Description

PIWIL2: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors. Interacts with DDX4, MAEL, EIF3A, EIF4E, EIF4G, PRMT5 and WDR77. Associates with EIF4E- and EIF4G-containing m7G cap-binding complexes. Interacts (when methylated on arginine residues) with TDRD1. Expressed in adult testis and in most tumors. Belongs to the argonaute family. Piwi subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA); Apoptosis

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: polysome; cytoplasm

Molecular Function: mRNA binding

Biological Process: oogenesis; meiotic cell cycle; positive regulation of translation; multicellular organismal development; DNA methylation during gametogenesis; spermatogenesis; germ-line stem cell maintenance; gene expression; RNA-mediated gene silencing