Typical Testing Data/Standard Curve (for reference only)
Guinea Pig INSL3 ELISA Kit | INSL3 elisa kit
Guinea pig INSL3 (Insulin Like Protein 3) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to INSL3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for INSL3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain INSL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of INSL3. You can calculate the concentration of INSL3 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the insulin-like hormone superfamily. The encoded protein is mainly produced in gonadal tissues. Studies of the mouse counterpart suggest that this gene may be involved in the development of urogenital tract and female fertility. This protein may also act as a hormone to regulate growth and differentiation of gubernaculum, and thus mediating intra-abdominal testicular descent. Mutations in this gene may lead to cryptorchidism. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]
Uniprot Description
INSL3: Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor. Defects in INSL3 seems to be a cause of cryptorchidism (CRYPTO); also known as impaired testicular descent. It is one of the most frequent congenital abnormalities in humans, involving 2-5% of male births. Cryptorchidism is associated with increased risk of infertility and testicular cancer. The frequency of INSL3 gene mutations as a cause of cryptorchidism is low. Belongs to the insulin family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 19p13.2-p12
Cellular Component: extracellular space; perinuclear region of cytoplasm; extracellular region
Molecular Function: protease binding; G-protein-coupled receptor binding; hormone activity; insulin receptor binding; receptor binding
Biological Process: negative regulation of cell proliferation; cell-cell signaling; in utero embryonic development; male gonad development; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; spermatogenesis; G-protein signaling, adenylate cyclase inhibiting pathway; oocyte maturation; negative regulation of apoptosis
Disease: Cryptorchidism, Unilateral Or Bilateral
Research Articles on INSL3
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Product Notes
The Guinea Pig INSL3 insl3 (Catalog #AAA2512477) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2512477 ELISA Kit recognizes Guinea Pig INSL3. It is sometimes possible for the material contained within the vial of "INSL3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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