Rat PRCP ELISA Kit | PRCP elisa kit

Rat PRCP (Prolylcarboxypeptidase) ELISA Kit

Cat. Num. AAA2502024

• Gene Names: Prcp; PCP; HUMPCP; AI451719; 2510048K03Rik; 2610104A14Rik
• Reactivity: Rat
• Synonyms: PRCP; Rat PRCP (Prolylcarboxypeptidase) ELISA Kit; PRCP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat PRCP. No significant cross-reactivity or interference between Rat PRCP and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat PRCP and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 491

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRCP elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PRCP in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PRCP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat PRCP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PRCP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat PRCP. You can calculate the concentration of Rat PRCP in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 32967631
• NCBI GeneID: 72461
• UniProt Accession #: Q7TMR0; Q3TLW3; Q3V302; Q8BKK3; Q8R0T8; Q9D049
• Molecular Weight: 11,803 Da
• NCBI Official Full Name: Prcp protein
• NCBI Official Synonym Full Names: prolylcarboxypeptidase (angiotensinase C)
• NCBI Official Symbol: Prcp
• NCBI Official Synonym Symbols: PCP; HUMPCP; AI451719; 2510048K03Rik; 2610104A14Rik
• NCBI Protein Information: lysosomal Pro-X carboxypeptidase; proline carboxypeptidase
• UniProt Protein Name: Lysosomal Pro-X carboxypeptidase
• Protein Family: Lysosomal Pro-X carboxypeptidase
• UniProt Gene Name: Prcp
• UniProt Synonym Gene Names: PRCP
• UniProt Entry Name: PCP_MOUSE

Uniprot Description

PRCP: Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. Belongs to the peptidase S28 family.

Protein type: Protease; EC 3.4.16.2

Cellular Component: lysosome

Molecular Function: peptidase activity; carboxypeptidase activity; hydrolase activity; serine-type peptidase activity

Biological Process: plasma kallikrein-kinin cascade; negative regulation of systemic arterial blood pressure; regulation of blood vessel endothelial cell migration; angiogenesis involved in wound healing; glucose homeostasis; proteolysis