RAB5A / RAB5 Polyclonal Antibody | anti-RAB5A / RAB5 antibody

Anti-RAB5A / RAB5 Antibody (C-Terminus) IHC-plus

Cat. Num. AAA249901

• Gene Names: RAB5A; RAB5
• Reactivity: Mouse, Dog, Rat, Human, Monkey
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Immunoaffinity purified
• Synonyms: RAB5A / RAB5; Polyclonal Antibody; Anti-RAB5A / RAB5 Antibody (C-Terminus) IHC-plus; Goat Polyclonal (IgG) to Mouse RAB5A / RAB5; Mouse RAB5A / RAB5; RAB5A; Ras-related protein Rab-5A; RAS-associated protein RAB5A; RAB5; anti-RAB5A / RAB5 antibody

• Host: RAB54A/RAB5 antibody was produced in Goat.
• Reactivity: Mouse, Dog, Rat, Human, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects total Rab5 protein in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab5a, GFP-Rab5b and GFP-Rab5c cds by Western blot.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, 20% glycerol, 0.05% sodium azide.
• Concentration: 3 mg/mL (varies by lot)
• Sequence Length: 215

• Applicable Applications for anti-RAB5A / RAB5 antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF (1:50 - 1:250); IHC-P (5 ug/ml); WB (1:250 - 1:2000); ; Optimal dilution to be determined by the researcher
• Immunogen: RAB5A / RAB5 antibody was raised against purified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5a, Rab5b and rab5c produced in E Coli.
• Immunogen Species: RAB5A/RAB5 antibody was raised against Mouse.
• Epitope: C-Terminus
• Target Species: Mouse
• Family/Subfamily: Ras GTPase superfamily IPR0011806/ RAS oncogene
• Preparation and Storage: Store at 4°short term.; Aliquot and store at -20°C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry - Paraffin (IHC)

(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry - Paraffin (IHC)

(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunofluorescence (IF)

(Immunofluorescence - anti-Rab5 antibody using B6-RPE07 cells at 1:50 dilution. Cells were fixed with 4% of PFA.)

Western Blot (WB)

(Western blot. Anti-Rab5 antibody at 1:1000 dilution. Tissue lysate at 100 ug per lane. Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.)

Related Product Information for anti-RAB5A / RAB5 antibody

RAB5A Antibody, Ras-related protein Rab-5A Antibody, RAS-associated protein RAB5A Antibody, RAB5 Antibody Description: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes.

NCBI and Uniprot Product Information

• NCBI GI #: 19923262
• NCBI GeneID: 5868
• NCBI Accession #: NP_004153.2
• NCBI GenBank Nucleotide #: NM_004162.4
• UniProt Accession #: P20339; Q6FI44; B4DJA5
• NCBI Official Full Name: ras-related protein Rab-5A isoform 1
• NCBI Official Synonym Full Names: RAB5A, member RAS oncogene family
• NCBI Official Symbol: RAB5A
• NCBI Official Synonym Symbols: RAB5
• NCBI Protein Information: ras-related protein Rab-5A
• UniProt Protein Name: Ras-related protein Rab-5A
• UniProt Gene Name: RAB5A
• UniProt Synonym Gene Names: RAB5
• UniProt Entry Name: RAB5A_HUMAN

Uniprot Description

RAB5A: Required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, RABEP1, SUN2, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; G protein, monomeric, Rab; Motility/polarity/chemotaxis; G protein

Chromosomal Location of Human Ortholog: 3p24-p22

Cellular Component: actin cytoskeleton; axon; cell soma; cytoplasm; cytosol; dendrite; early endosome; endocytic vesicle; endosome; endosome membrane; lipid raft; melanosome; nerve terminal; phagocytic vesicle; plasma membrane; ruffle; synaptic vesicle; terminal button

Molecular Function: GDP binding; GTP binding; GTPase activity; protein binding

Biological Process: blood coagulation; early endosome to late endosome transport; endocytosis; positive regulation of exocytosis; protein transport; regulation of endocytosis; regulation of endosome size; regulation of filopodium formation; small GTPase mediated signal transduction