Western Blot (WB)
(Western blot detection of Rab5 in K562,C6,3T3,Hela cell lysates using Rab5 Rabbit pAb(1:1000 diluted). Predicted band size:24kDa. Observed band size:24kDa.)
Rabbit Rab5 Polyclonal Antibody | anti-RAB5A antibody
Rab5 Rabbit pAb
Gene Names
RAB5A; RAB5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
Rab5; Polyclonal Antibody; Rab5 Rabbit pAb; Ras-related protein Rab-5A; RAB5; anti-RAB5A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-RAB5A antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000
IHC: 1:50
IHC: 1:50
Immunogen
A synthetic peptide of human Rab5
Conjugation
Unconjugated
Modification
Unmodification
Subcellular Location
Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot detection of Rab5 in K562,C6,3T3,Hela cell lysates using Rab5 Rabbit pAb(1:1000 diluted). Predicted band size:24kDa. Observed band size:24kDa.)
Western Blot (WB)
(Western blot detection of Rab5 in K562,C6,3T3,Hela cell lysates using Rab5 Rabbit pAb(1:1000 diluted). Predicted band size:24kDa. Observed band size:24kDa.)
Related Product Information for anti-RAB5A antibody
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
215
NCBI Official Full Name
ras-related protein Rab-5A
NCBI Official Synonym Full Names
RAB5A, member RAS oncogene family
NCBI Official Symbol
RAB5A
NCBI Official Synonym Symbols
RAB5
NCBI Protein Information
ras-related protein Rab-5A; RAS-associated protein RAB5A
UniProt Protein Name
Ras-related protein Rab-5A
Protein Family
UniProt Gene Name
RAB5A
UniProt Synonym Gene Names
RAB5
UniProt Entry Name
RAB5A_HUMAN
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Product Notes
The RAB5A rab5a (Catalog #AAA4752202) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rab5 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Rab5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000 IHC: 1:50. Researchers should empirically determine the suitability of the RAB5A rab5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rab5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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