Mouse anti-Human ATP5E Monoclonal Antibody | anti-ATP5E antibody

Mouse Monoclonal [clone 2F3] (IgG1,k) to Human ATP5E

Cat. Num. AAA245926

• Gene Names: ATP5E; ATPE; MC5DN3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: ATP5E; Monoclonal Antibody; Mouse Monoclonal [clone 2F3] (IgG1; k) to Human ATP5E; Anti-ATP5E Antibody (clone 2F3) IHC-plus; ATPE; ATP synthase epsilon chain; ATPase subunit epsilon; MC5DN3; Mitochondrial ATPase; F(0)F(1)-ATPase; Human ATP5E; anti-ATP5E antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2F3
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.36 mg/ml (varies by lot)
• Sequence Length: 51

• Applicable Applications for anti-ATP5E antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Western Blot (Cell lysate) - positive control SW-13.
• Target Species: Human
• Immunogen Description: ATP5E (AAH01690, 1 a.a. ~ 52 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: ATP5E antibody was raised against aTP5E (AAH01690, 1 a.a. ~ 52 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-ATP5E antibody IHC of human liver, bile ducts. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ATP5E antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ATP5E antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(ATP5E monoclonal antibody, clone 2F3 Western blot of ATP5E expression in SW-13.)

Product Categories/Family for anti-ATP5E antibody

Family: Transporter; Subfamily: ATPase - F type

NCBI and Uniprot Product Information

• NCBI GI #: 5901896
• NCBI GeneID: 514
• NCBI Accession #: NP_008817.1
• NCBI GenBank Nucleotide #: NM_006886.3
• UniProt Accession #: P56381; Q53XU6; B2RDD0; E1P5H6
• Molecular Weight: 5,780 Da
• NCBI Official Full Name: ATP synthase subunit epsilon, mitochondrial
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
• NCBI Official Symbol: ATP5E
• NCBI Official Synonym Symbols: ATPE; MC5DN3
• NCBI Protein Information: ATP synthase subunit epsilon, mitochondrial; F(0)F(1)-ATPase; mitochondrial ATPase; H(+)-transporting two-sector ATPase; mitochondrial ATP synthase epsilon chain
• UniProt Protein Name: ATP synthase subunit epsilon, mitochondrial
• UniProt Gene Name: ATP5E
• UniProt Synonym Gene Names: ATPase subunit epsilon
• UniProt Entry Name: ATP5E_HUMAN

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the epsilon subunit of the catalytic core. Two pseudogenes of this gene are located on chromosomes 4 and 13. Read-through transcripts that include exons from this gene are expressed from the upstream gene SLMO2.[provided by RefSeq, Mar 2011]

Uniprot Description

ATP5E: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Defects in ATP5E are the cause of mitochondrial complex V deficiency nuclear type 3 (MC5DN3). MC5DN3 is a mitochondrial disorder with heterogeneous clinical manifestations including dysmorphic features, psychomotor retardation, hypotonia, growth retardation, cardiomyopathy, enlarged liver, hypoplastic kidneys and elevated lactate levels in urine, plasma and cerebrospinal fluid. Belongs to the eukaryotic ATPase epsilon family.

Protein type: Mitochondrial; Transporter; Hydrolase; Energy Metabolism - oxidative phosphorylation; Membrane protein, integral; EC 3.6.3.14

Chromosomal Location of Human Ortholog: 20q13.32

Cellular Component: mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex

Molecular Function: ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; transmembrane transporter activity; hydrogen ion transporting ATPase activity, rotational mechanism

Biological Process: cellular metabolic process; mitochondrial ATP synthesis coupled proton transport

Disease: Mitochondrial Complex V (atp Synthase) Deficiency, Nuclear Type 3