Rabbit ATP5E Polyclonal Antibody | anti-ATP5E antibody

ATP5E Antibody

Cat. Num. AAA9609555

• Gene Names: ATP5F1E; ATPE; ATP5E; MC5DN3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Peptide affinity purification
• Synonyms: ATP5E; Polyclonal Antibody; ATP5E Antibody; anti-ATP5E antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATP5E antibody detects endogenous levels of total ATP5E
• Purity/Purification: Peptide affinity purification
• Form/Format: Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 51

• Applicable Applications for anti-ATP5E antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IHC: 1:200; ELISA(peptide): 1:20000-1:40000; ; The optimal dilutions should be determined by the enduser.
• Immunogen: A synthesized peptide derived from human ATP5E, corresponding to a region within the internal amino acids.
• IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Preparation and Storage: Store at -20°C.

Western Blot (WB)

(Western blot analysis of ATP5E using HT29 whole cell lysates)

Immunohistochemistry (IHC)

(MBS9609555 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-ATP5E antibody

Function: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1-containing the extramembraneous catalytic core, and F0-containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F1 domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits (By similarity).
Subunit Structure: F-type ATPases have 2 components, CF1-the catalytic core-and CF0-the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 seems to have nine subunits: a, b, c, d, e, f, g, F6 and 8 (or A6L). Component of an ATP synthase complex composed of ATP5F1, ATP5MC1, ATP5F1E, ATP5H, ATP5I, ATP5J, ATP5J2, MT-ATP6, MT-ATP8, ATP5F1A, ATP5F1B, ATP5F1D, ATP5F1C, ATP5O, ATP5L, USMG5 and MP68 (By similarity).
Similarity: Belongs to the eukaryotic ATPase epsilon family.

NCBI and Uniprot Product Information

• NCBI GI #: 5901896
• NCBI GeneID: 514
• NCBI Accession #: NP_008817.1
• NCBI GenBank Nucleotide #: NM_006886.3
• UniProt Accession #: P56381; Q53XU6; B2RDD0; E1P5H6
• Molecular Weight: 6kDa
• NCBI Official Full Name: ATP synthase subunit epsilon, mitochondrial
• NCBI Official Synonym Full Names: ATP synthase F1 subunit epsilon
• NCBI Official Symbol: ATP5F1E
• NCBI Official Synonym Symbols: ATPE; ATP5E; MC5DN3
• NCBI Protein Information: ATP synthase subunit epsilon, mitochondrial
• UniProt Protein Name: ATP synthase subunit epsilon, mitochondrial
• UniProt Gene Name: ATP5F1E
• UniProt Synonym Gene Names: ATP5EImportedManual assertion inferred from database entriesiHGNC:838; ATPase subunit epsilon

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the epsilon subunit of the catalytic core. Two pseudogenes of this gene are located on chromosomes 4 and 13. Read-through transcripts that include exons from this gene are expressed from the upstream gene SLMO2.[provided by RefSeq, Mar 2011]

Uniprot Description

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F1 domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits ().