Rabbit PUMA Polyclonal Antibody | anti-PUMA antibody

Rabbit Polyclonal (IgG) to Human PUMA

Cat. Num. AAA243445

• Gene Names: BBC3; JFY1; PUMA; JFY-1
• Reactivity: Gibbon, Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: PUMA; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human PUMA; Anti-PUMA Antibody (N-Terminus) IHC-plus; BBC3; Bcl-2-binding component 3; BCL2 binding component 3; JFY-1; JFY1; Human PUMA; anti-PUMA antibody

• Host: Rabbit
• Reactivity: Gibbon, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the p53 - upregulated modulator of apoptosis (PUMA), also known as bbc3. PUMA is a pro-apoptotic member of the BCL-2 family. PUMA is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53
• Purity/Purification: Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 193

• Applicable Applications for anti-PUMA antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (2.5 ug/ml), WB (2 - 4 ug/ml); Usage: Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. Histology Positive Control Tissue: Human breast carcinoma. Western...
• Target Species: Human
• Immunogen Description: Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human PUMA alpha. Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Porcine (100%); Opossum (87%).
• Immunogen Type: Synthetic peptide
• Immunogen: PUMA antibody was raised against synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human PUMA alpha. Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Porcine (100%); Opossum (87%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-BBC3 / PUMA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Related Product Information for anti-PUMA antibody

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53.

Product Categories/Family for anti-PUMA antibody

Family: Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 15193488
• NCBI GeneID: 27113
• NCBI Accession #: NP_055232.1
• NCBI GenBank Nucleotide #: NM_014417.4
• UniProt Accession #: Q9BXH1; O00171; Q96PG9; B9EGI3; Q96PG8
• Molecular Weight: 10,326 Da
• NCBI Official Full Name: bcl-2-binding component 3 isoform 4
• NCBI Official Synonym Full Names: BCL2 binding component 3
• NCBI Official Symbol: BBC3
• NCBI Official Synonym Symbols: JFY1; PUMA; JFY-1
• NCBI Protein Information: bcl-2-binding component 3; p53 up-regulated modulator of apoptosis
• UniProt Protein Name: Bcl-2-binding component 3
• UniProt Gene Name: BBC3
• UniProt Synonym Gene Names: PUMA
• UniProt Entry Name: BBC3_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

Uniprot Description

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; determination of adult life span; response to DNA damage stimulus; negative regulation of growth