Rabbit BCL2 / Bcl-2 Polyclonal Antibody | anti-BCL2 antibody

Rabbit Polyclonal (IgG) to Human BCL2 / Bcl-2

Cat. Num. AAA242899

• Gene Names: BCL2; Bcl-2; PPP1R50
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: BCL2 / Bcl-2; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human BCL2 / Bcl-2; BCL2 Antibody; Apoptosis regulator Bcl-2 Antibody; B-cell CLL/lymphoma 2 Antibody; B-cell lymphoma protein 2 Antibody; Bcl-2 Antibody; PPP1R50 Antibody; anti-BCL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BCL-2 (Ab-69) Antibody detects endogenous levels of total BCL-2 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Sequence Length: 239

• Applicable Applications for anti-BCL2 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000), IF (1:100 - 1:500), IHC-P (1:100), WB (1:500 - 1:1000); Optimal dilution to be determined by the researcher.
• Target Species: Human
• Immunogen Description: Synthetic peptide from human BCL-2 (aa46-95).
• Antigen Type: Synthetic peptide
• Immunogen: BCL2 / Bcl-2 antibody was raised against synthetic peptide from human BCL-2 (aa46-95).
• Antigen Modification: aa46-95
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-BCL2 / Bcl-2 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Immunofluorescence (IF)

(Immunofluorescence of HepG2 cells, using BCL-2 (Ab-69) Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from K562 cells, using BCL-2 (Ab-69) Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-BCL2 antibody

Bcl-2 inhibits apoptosis. Translocations that lead to overexpression of this gene are associated with cancer (lymphoma in the case of translocation to the Ig heavy chain locus).

Product Categories/Family for anti-BCL2 antibody

Family: Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 72198189
• NCBI GeneID: 596
• NCBI Accession #: NP_000624.2
• NCBI GenBank Nucleotide #: NM_000633.2
• UniProt Accession #: P10415; P10416; Q13842; Q16197; C9JHD5
• Molecular Weight: 22,337 Da
• NCBI Official Full Name: apoptosis regulator Bcl-2 alpha isoform
• NCBI Official Synonym Full Names: B-cell CLL/lymphoma 2
• NCBI Official Symbol: BCL2
• NCBI Official Synonym Symbols: Bcl-2; PPP1R50
• NCBI Protein Information: apoptosis regulator Bcl-2; protein phosphatase 1, regulatory subunit 50
• UniProt Protein Name: Apoptosis regulator Bcl-2
• Protein Family: Apoptosis regulator
• UniProt Gene Name: BCL2
• UniProt Entry Name: BCL2_HUMAN

NCBI Description

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]

Uniprot Description

Bcl-2: a antiapoptotic member of the Bcl-2 family. Regulates cell death by controlling the mitochondrial membrane permeability. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Phosphorylation by JNKs may increase its antiapoptotic functions.

Protein type: Autophagy; Apoptosis; Membrane protein, integral; Oncoprotein

Chromosomal Location of Human Ortholog: 18q21.3

Cellular Component: pore complex; endoplasmic reticulum membrane; mitochondrial outer membrane; nuclear membrane; mitochondrion; membrane; endoplasmic reticulum; cytoplasm; cytosol; nucleus; myelin sheath

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protease binding; protein phosphatase 2A binding; channel activity; sequence-specific DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; BH3 domain binding; channel inhibitor activity; transcription factor binding

Biological Process: response to nicotine; focal adhesion formation; positive regulation of catalytic activity; developmental growth; renal system process; pigment granule organization and biogenesis; protein polyubiquitination; response to toxin; response to glucocorticoid stimulus; T cell differentiation in the thymus; ear development; lymphoid progenitor cell differentiation; positive regulation of multicellular organism growth; female pregnancy; glomerulus development; negative regulation of mitochondrial depolarization; post-embryonic development; cochlear nucleus development; cellular response to glucose starvation; B cell receptor signaling pathway; negative regulation of myeloid cell apoptosis; regulation of mitochondrial membrane potential; negative regulation of ossification; positive regulation of B cell proliferation; regulation of transmembrane transporter activity; T cell homeostasis; negative regulation of neuron apoptosis; cell growth; defense response to virus; response to drug; spleen development; positive regulation of neuron maturation; release of cytochrome c from mitochondria; regulation of protein homodimerization activity; axon regeneration; cell aging; actin filament organization; digestive tract morphogenesis; regulation of calcium ion transport; positive regulation of cell growth; organ growth; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; gland morphogenesis; negative regulation of osteoblast proliferation; regulation of mitochondrial membrane permeability; regulation of nitrogen utilization; metanephros development; oocyte development; negative regulation of apoptosis; B cell proliferation; negative regulation of autophagy; regulation of protein heterodimerization activity; behavioral fear response; melanin metabolic process; negative regulation of retinal cell programmed cell death; regulation of cell-matrix adhesion; apoptosis; regulation of protein stability; positive regulation of smooth muscle cell migration; protein amino acid dephosphorylation; response to radiation; positive regulation of skeletal muscle fiber development; ovarian follicle development; B cell homeostasis; positive regulation of melanocyte differentiation; melanocyte differentiation; response to gamma radiation; transmembrane transport; negative regulation of cell migration; response to iron ion; negative regulation of cellular pH reduction; regulation of viral genome replication; mesenchymal cell development; ossification; hair follicle morphogenesis; CD8-positive, alpha-beta T cell lineage commitment; thymus development; B cell lineage commitment; male gonad development; peptidyl-threonine phosphorylation; positive regulation of peptidyl-serine phosphorylation; humoral immune response; response to UV-B; endoplasmic reticulum calcium ion homeostasis; peptidyl-serine phosphorylation; neuron apoptosis; response to hydrogen peroxide; axonogenesis; ureteric bud branching; homeostasis of number of cells within a tissue; response to cytokine stimulus; innate immune response; negative regulation of cell growth; response to DNA damage stimulus; induction of apoptosis by oxidative stress