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Western Blot (WB) (Western blot analysis of Citrate synthetase on Hela cell using anti-Citrate synthetase antibody at 1/1, 000 dilution. Positive control: Lane 1: Rat kidney tissue Lane 2: Hela Lane 3: Mouse brain tissue)

Rabbit Citrate Synthetase Monoclonal Antibody | anti-CS antibody

Citrate Synthetase Antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Citrate Synthetase; Monoclonal Antibody; Citrate Synthetase Antibody; CISY_HUMAN antibody; Citrate synthase antibody; Citrate synthase; mitochondrial antibody; citrate synthetase antibody; Cs antibody; EC 2.3.3 antibody; EC 2.3.3.1 antibody; anti-CS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JU80-30
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CS antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
FC/FACS: 1:50-1:200
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Citrate synthetase on Hela cell using anti-Citrate synthetase antibody at 1/1, 000 dilution. Positive control: Lane 1: Rat kidney tissue Lane 2: Hela Lane 3: Mouse brain tissue)

Western Blot (WB) (Western blot analysis of Citrate synthetase on Hela cell using anti-Citrate synthetase antibody at 1/1, 000 dilution. Positive control: Lane 1: Rat kidney tissue Lane 2: Hela Lane 3: Mouse brain tissue)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Citrate synthetase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Citrate synthetase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Citrate synthetase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Citrate synthetase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Citrate synthetase antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Citrate synthetase antibody. Counter stained with hematoxylin.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293T cells with Citrate synthetase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of 293T cells with Citrate synthetase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-CS antibody
Citrate synthase is found in nearly all cells capable of oxidative metabolism.The enzyme citrate synthase exists in nearly all living cells and stands as a pace-making enzyme in the first step of the citric acid cycle (or Krebs cycle). Citrate synthase is localized within eukaryotic cells in the mitochondrial matrix, but is encoded by nuclear DNA rather than mitochondrial. It is synthesized using cytoplasmic ribosomes, then transported into the mitochondrial matrix. Citrate synthase is commonly used as a quantitative enzyme marker for the presence of intact mitochondria.
Product Categories/Family for anti-CS antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,712 Da
NCBI Official Full Name
citrate synthase, mitochondrial
NCBI Official Synonym Full Names
citrate synthase
NCBI Official Symbol
CS
NCBI Protein Information
citrate synthase, mitochondrial
UniProt Protein Name
Citrate synthase, mitochondrial
UniProt Gene Name
CS
UniProt Entry Name
CISY_HUMAN

Uniprot Description

CS: a mitochondrial enzyme of the tricarboxylic acid cycle that converts acetyl-CoA + H2O + oxaloacetate into citrate + CoA. Found in nearly all cells capable of oxidative metabolism.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 2.3.3.1; Mitochondrial; Transferase

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: mitochondrial matrix; mitochondrion; nucleus

Molecular Function: citrate (Si)-synthase activity

Biological Process: carbohydrate metabolic process; tricarboxylic acid cycle

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Product Notes

The CS cs (Catalog #AAA9436466) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Citrate Synthetase Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Citrate Synthetase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 IHC: 1:50-1:100 FC/FACS: 1:50-1:200. Researchers should empirically determine the suitability of the CS cs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Citrate Synthetase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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