Rabbit anti-Mouse, Human PRG2 / Proteoglycan 2 Polyclonal Antibody | anti-PRG2 antibody

Anti-PRG2 / Proteoglycan 2 Antibody IHC-plus

Cat. Num. AAA249802

• Gene Names: PRG2; MBP; BMPG; MBP1
• Reactivity: Mouse, Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Affinity purified
• Synonyms: PRG2 / Proteoglycan 2; Polyclonal Antibody; Anti-PRG2 / Proteoglycan 2 Antibody IHC-plus; PRG2; BMPG; Bone marrow proteoglycan; Bone-marrow proteoglycan; Eosinophil major basic protein; MBP1; Natural killer cell activator; Proteoglycan 2; Proteoglycan 2 preproprotein; anti-PRG2 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human PRG2 / Proteoglycan 2
• Purity/Purification: Affinity purified
• Form/Format: PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
• Sequence Length: 222

• Applicable Applications for anti-PRG2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC (1:50 - 1:200); IHC - Paraffin (1:200); Western blot (1:500 - 1:2000); (optimal dilution to be determined by the researcher)
• Target: PRG2/Proteoglycan 2
• Immunogen Species: PRG2 / Proteoglycan 2 antibody was raised against Human
• Immunogen: PRG2 / Proteoglycan 2 antibody was raised against recombinant human PRG2.
• Immunogen Description: Recombinant human PRG2.
• Antigen Type: Recombinant protein
• Target Species: Human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Upon arrival: Store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry - Paraffin (IHC)

(Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PRG2 antibody.)

Related Product Information for anti-PRG2 antibody

PRG2 Antibody, BMPG Antibody, Bone marrow proteoglycan Antibody, Bone-marrow proteoglycan Antibody, Eosinophil major basic protein Antibody, MBP1 Antibody, Natural killer cell activator Antibody, Proteoglycan 2 Antibody, Proteoglycan 2 preproprotein Antibody Description: PRG2 / Proteoglycan 2 is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions.

NCBI and Uniprot Product Information

• NCBI GI #: 46276889
• NCBI GeneID: 5553
• NCBI Accession #: NP_002719.3
• NCBI GenBank Nucleotide #: NM_002728.5
• UniProt Accession #: P13727; P81448; Q14227; Q6ICT2; A6XMW0; B2R5I1
• Molecular Weight: 23,901 Da
• NCBI Official Full Name: bone marrow proteoglycan isoform 1 preproprotein
• NCBI Official Synonym Full Names: proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
• NCBI Official Symbol: PRG2
• NCBI Official Synonym Symbols: MBP; BMPG; MBP1
• NCBI Protein Information: bone marrow proteoglycan
• UniProt Protein Name: Bone marrow proteoglycan
• Protein Family: Bone marrow proteoglycan
• UniProt Gene Name: PRG2
• UniProt Synonym Gene Names: MBP; BMPG; EMBP; MBP
• UniProt Entry Name: PRG2_HUMAN

NCBI Description

The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. The encoded protein contains a peptide that displays potent antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria, and fungi. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

Uniprot Description

Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.