Rabbit PARK7 / DJ-1 Polyclonal Antibody | anti-PARK7 antibody

Rabbit Polyclonal to Human PARK7 / DJ-1

Cat. Num. AAA241363

• Gene Names: PARK7; DJ1; DJ-1; HEL-S-67p
• Reactivity: African green monkey, Chimpanzee, Human, Zebrafish
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: PARK7 / DJ-1; Polyclonal Antibody; Rabbit Polyclonal to Human PARK7 / DJ-1; Anti-PARK7 / DJ-1 Antibody (aa177-189) IHC-plus; PARK7; DJ-1; DJ1; Parkinson disease protein 7; Park-7; Oncogene DJ1; Parkinson protein 7; Protein DJ-1; Human PARK7; anti-PARK7 antibody

• Host: Rabbit
• Reactivity: African green monkey, Chimpanzee, Human, Zebrafish
• Clonality: Polyclonal
• Specificity: aa 177-189 of Human PARK7 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 189

• Applicable Applications for anti-PARK7 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000 - 1:50000), IHC-P (2.5 ug/ml), WB (1:500 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human PARK7 / DJ-1.
• Immunogen Type: Synthetic peptide
• Immunogen: PARK7 / DJ-1 antibody was raised against synthetic peptide from human PARK7 / DJ-1.
• Antigen Modification: aa177-189
• Preparation and Storage: Store vial at -20 degree C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PARK7 / DJ-1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-Human PARK7 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Human PARK7 antibody to detect PARK7 present in Jurkat whole cell lysate. This western blot shows reactivity with human PARK7 protein. Comparison to a molecular weight marker indicates a predominant band of ~28.0 kD. Peptide competition blocks specific reactivity of the antibody with PARK7 (not shown). A 16% Tris-Tricine gel was used to separate proteins prior to transfer to 0.2 micron nitrocellulose. The blot was incubated with a 1:1300 dilution of the antibody overnight at 4C followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] (diluted 1:5000 for 45 min at RT. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)

NCBI and Uniprot Product Information

• NCBI GI #: 31543380
• NCBI GeneID: 11315
• NCBI Accession #: NP_009193.2
• NCBI GenBank Nucleotide #: NM_007262.4
• UniProt Accession #: Q99497; O14805; Q6DR95; Q7LFU2; B2R4Z1
• Molecular Weight: 19,891 Da
• NCBI Official Full Name: protein DJ-1
• NCBI Official Synonym Full Names: parkinson protein 7
• NCBI Official Symbol: PARK7
• NCBI Official Synonym Symbols: DJ1; DJ-1; HEL-S-67p
• NCBI Protein Information: protein DJ-1; oncogene DJ1; epididymis secretory sperm binding protein Li 67p; Parkinson disease (autosomal recessive, early onset) 7
• UniProt Protein Name: Protein DJ-1
• Protein Family: Protein deglycase
• UniProt Gene Name: PARK7
• UniProt Entry Name: PARK7_HUMAN

NCBI Description

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration.

Protein type: EC 3.4.-.-; Transcription regulation; Tumor suppressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: PML body; mitochondrion; mitochondrial matrix; axon; cytoplasm; plasma membrane; mitochondrial intermembrane space; nucleus; mitochondrial respiratory chain complex I; chromatin; cytosol; lipid raft

Molecular Function: identical protein binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; mercury ion binding; transcription coactivator activity; transcription factor binding; peptidase activity; mRNA binding; protein binding; enzyme binding; copper ion binding; androgen receptor binding; peroxiredoxin activity; cytokine binding; double-stranded DNA binding; glyoxalase III activity; superoxide dismutase copper chaperone activity; kinase binding; single-stranded DNA binding; receptor binding

Biological Process: negative regulation of protein export from nucleus; adult locomotory behavior; protein deglycosylation; membrane hyperpolarization; proteolysis; glycolate biosynthetic process; positive regulation of interleukin-8 production; regulation of mitochondrial membrane potential; negative regulation of protein amino acid phosphorylation; single fertilization; regulation of neuron apoptosis; enzyme active site formation via L-cysteine sulfinic acid; dopamine uptake; negative regulation of neuron apoptosis; inflammatory response; negative regulation of protein binding; mitochondrion organization and biogenesis; protein stabilization; activation of protein kinase B; regulation of TRAIL receptor biosynthetic process; detoxification of copper ion; positive regulation of peptidyl-serine phosphorylation; negative regulation of protein sumoylation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; methylglyoxal catabolic process to D-lactate; membrane depolarization; positive regulation of protein kinase B signaling cascade; detoxification of mercury ion; Ras protein signal transduction; regulation of inflammatory response; hydrogen peroxide metabolic process; negative regulation of ubiquitin-protein ligase activity; lactate biosynthetic process; autophagy; negative regulation of protein kinase activity; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein ubiquitination; negative regulation of apoptosis

Disease: Parkinson Disease 7, Autosomal Recessive Early-onset