Goat PARK7 / DJ-1 Polyclonal Antibody | anti-PARK7 antibody

Goat Polyclonal to Human PARK7 / DJ-1

Cat. Num. AAA244781

• Gene Names: PARK7; DJ1; DJ-1; HEL-S-67p
• Reactivity: Gibbon, Gorilla, Human, Monkey; Predicted Reactivity: Mouse, Rat, Bovine, Dog, Hamster, Horse, Rabbit, Zebrafish (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: PARK7 / DJ-1; Polyclonal Antibody; Goat Polyclonal to Human PARK7 / DJ-1; Anti-PARK7 / DJ-1 Antibody (C-Terminus) IHC-plus; PARK7; DJ-1; DJ1; Parkinson disease protein 7; Park-7; Oncogene DJ1; Parkinson protein 7; Protein DJ-1; Human PARK7; anti-PARK7 antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human, Monkey; Predicted Reactivity: Mouse, Rat, Bovine, Dog, Hamster, Horse, Rabbit, Zebrafish (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human PARK7 / DJ-1. Variants (NP_001116849.1; NP_009193.2) encode the same protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 189

• Applicable Applications for anti-PARK7 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (3.75 ug/ml), WB (0.5 - 1 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide C-AAQVKAPLVLKD from the C-terminus of human PARK7 / DJ-1 (NP_001116849.1; NP_009193.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: PARK7 / DJ-1 antibody was raised against synthetic peptide C-AAQVKAPLVLKD from the C-terminus of human PARK7 / DJ-1 (NP_001116849.1; NP_009193.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Salmon, Stickleback, Zebrafish (92%); Bat, Pig, Turkey, Chicken, Medaka, Pike (83%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PARK7 / DJ-1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-PARK7 / DJ-1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 31543380
• NCBI GeneID: 11315
• NCBI Accession #: NP_009193.2
• NCBI GenBank Nucleotide #: NM_007262.4
• UniProt Accession #: Q99497; O14805; Q6DR95; Q7LFU2; B2R4Z1
• Molecular Weight: 19,891 Da
• NCBI Official Full Name: protein DJ-1
• NCBI Official Synonym Full Names: parkinson protein 7
• NCBI Official Symbol: PARK7
• NCBI Official Synonym Symbols: DJ1; DJ-1; HEL-S-67p
• NCBI Protein Information: protein DJ-1; oncogene DJ1; epididymis secretory sperm binding protein Li 67p; Parkinson disease (autosomal recessive, early onset) 7
• UniProt Protein Name: Protein DJ-1
• Protein Family: Protein deglycase
• UniProt Gene Name: PARK7
• UniProt Entry Name: PARK7_HUMAN

NCBI Description

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration.

Protein type: EC 3.4.-.-; Transcription regulation; Nuclear receptor co-regulator; Tumor suppressor

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: PML body; mitochondrion; mitochondrial matrix; axon; cytoplasm; plasma membrane; mitochondrial intermembrane space; nucleus; chromatin; cytosol; mitochondrial respiratory chain complex I; lipid raft

Molecular Function: identical protein binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; transcription coactivator activity; mercury ion binding; transcription factor binding; mRNA binding; peptidase activity; protein binding; copper ion binding; enzyme binding; peroxiredoxin activity; androgen receptor binding; cytokine binding; double-stranded DNA binding; glyoxalase III activity; superoxide dismutase copper chaperone activity; kinase binding; single-stranded DNA binding; receptor binding

Biological Process: negative regulation of protein export from nucleus; adult locomotory behavior; protein deglycosylation; membrane hyperpolarization; proteolysis; glycolate biosynthetic process; positive regulation of interleukin-8 production; negative regulation of protein amino acid phosphorylation; regulation of mitochondrial membrane potential; single fertilization; regulation of neuron apoptosis; enzyme active site formation via L-cysteine sulfinic acid; dopamine uptake; negative regulation of neuron apoptosis; negative regulation of protein binding; inflammatory response; mitochondrion organization and biogenesis; protein stabilization; activation of protein kinase B; regulation of TRAIL receptor biosynthetic process; detoxification of copper ion; negative regulation of protein sumoylation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of peptidyl-serine phosphorylation; methylglyoxal catabolic process to D-lactate; positive regulation of protein kinase B signaling cascade; membrane depolarization; detoxification of mercury ion; Ras protein signal transduction; regulation of inflammatory response; hydrogen peroxide metabolic process; negative regulation of ubiquitin-protein ligase activity; lactate biosynthetic process; negative regulation of protein kinase activity; autophagy; positive regulation of transcription factor activity; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; negative regulation of apoptosis

Disease: Parkinson Disease 7, Autosomal Recessive Early-onset