Catenin Beta 1 (CTNNb1) Recombinant Protein | CTNNb1 recombinant protein

Recombinant Catenin Beta 1 (CTNNb1)

Cat. Num. AAA2009008

• Gene Names: Ctnnb1; Catnb
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Catenin Beta 1 (CTNNb1); Recombinant Catenin Beta 1 (CTNNb1); CTNNb1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-LG SDDINVVTCA AGILSNLTCN NYKNKMMVCQ VGGIEALVRT VLRAGDREDI TEPAICALRH LTSRHQEAEM AQNAVRLHYG LPVVVKLLHP PSHWPLIKAT VGLIRNLALC PANHAPLREQ GAIPRLVQLL VRAHQDTQRR TSMGGTQQQF VEGVRMEEIV EGCTGALHIL ARDVHNRIVI RGLNTIPLFV QLLYSPIENI QRVAAGVLCE LAQDKEAAEA IEAEGATAPL TELLHSRNEG VATYAAAVLF RMSED
• Sequence Length: 781

• Applicable Applications for CTNNb1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Leu409~Asp665 (Accession # Q9WU82) with N-terminal His-Tag
• Subcellular Location: Cytoplasm. cytoskeleton. Nucleus. Cell junction, adherens junction. Cell membrane. centrosome. spindle pole
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.6
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 46048609
• NCBI GeneID: 84353
• NCBI Accession #: NP_445809.2
• NCBI GenBank Nucleotide #: NM_053357.2
• UniProt Accession #: Q9WU82
• Molecular Weight: 29.3kDa
• NCBI Official Full Name: catenin beta-1
• NCBI Official Synonym Full Names: catenin (cadherin associated protein), beta 1
• NCBI Official Symbol: Ctnnb1
• NCBI Official Synonym Symbols: Catnb
• NCBI Protein Information: catenin beta-1; beta-catenin; catenin (cadherin associated protein), beta 1, 88kDa
• UniProt Protein Name: Catenin beta-1
• Protein Family: Catenin
• UniProt Gene Name: Ctnnb1
• UniProt Synonym Gene Names: Catnb
• UniProt Entry Name: CTNB1_RAT

NCBI Description

involved in mediating the interaction between cadherins and the actin cytoskeleton [RGD, Feb 2006]

Uniprot Description

CTNNB1: a regulator of cell adhesion and a key downstream effector in the Wnt signaling pathway. Implicated early embryonic development and tumorigenesis. Phosphorylated and destabilized by CK1 and GSK-3beta. Stabilized cytoplasmic beta-catenin is a hallmark of a variety of cancers. Stabilized beta-catenin translocates to the nucleus, where it acts as a transcriptional activator of T-cell factor (TCF)-regulated genes. Interacts with the PDZ domain of TAX1BP3, inhibiting its transcriptional activity. Two alternatively spliced human isoforms have been described.

Protein type: Actin-binding; Nuclear receptor co-regulator; Transcription factor; Oncoprotein; Motility/polarity/chemotaxis; Cell adhesion

Cellular Component: centrosome; basolateral plasma membrane; fascia adherens; intercellular junction; cytosol; beta-catenin destruction complex; transcription factor complex; cell-cell adherens junction; membrane; cell projection membrane; perinuclear region of cytoplasm; lamellipodium; cytoplasm; synapse; dendritic shaft; lateral plasma membrane; apical junction complex; spindle pole; neuron projection; focal adhesion; protein complex; tight junction; cell; catenin complex; cell cortex; Z disc; adherens junction; microvillus membrane; apical part of cell; plasma membrane; nucleus; cell junction

Molecular Function: protein C-terminus binding; transcription coactivator activity; transcription factor binding; protein phosphatase binding; ionotropic glutamate receptor binding; signal transducer activity; protein binding; enzyme binding; DNA binding; cadherin binding; double-stranded DNA binding; protein complex binding; estrogen receptor binding; chromatin binding; nitric-oxide synthase binding; SMAD binding; transcription factor activity; kinase binding; alpha-catenin binding; nuclear hormone receptor binding

Biological Process: skin development; cellular morphogenesis during differentiation; regulation of myelination; regulation of centriole-centriole cohesion; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; cell maturation; negative regulation of chondrocyte differentiation; positive regulation of fibroblast growth factor receptor signaling pathway; cell fate determination; morphogenesis of embryonic epithelium; chromatin-mediated maintenance of transcription; embryonic foregut morphogenesis; regulation of cell fate specification; positive regulation of mesenchymal cell proliferation; male genitalia development; synapse organization and biogenesis; kidney development; T cell differentiation; response to drug; positive regulation of neuroblast proliferation; transcription, DNA-dependent; regulation of osteoblast differentiation; regulation of smooth muscle cell proliferation; regulation of transcription from RNA polymerase II promoter; response to estrogen stimulus; regulation of gene expression; regulation of epithelial cell differentiation; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; limb development; embryonic forelimb morphogenesis; neuron migration; response to hormone stimulus; dorsal/ventral axis specification; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; pancreas development; forebrain development; vasculogenesis; proximal/distal pattern formation; negative regulation of mitotic cell cycle, embryonic; Wnt receptor signaling pathway; in utero embryonic development; neural plate development; gastrulation with mouth forming second; positive regulation of telomere maintenance via telomerase; regulation of cell proliferation; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; Schwann cell proliferation; cell proliferation; negative regulation of cell differentiation; response to cadmium ion; dorsal/ventral pattern formation; ureteric bud branching; embryonic heart tube development; lens morphogenesis in camera-type eye; lung development; anterior/posterior axis specification; protein heterooligomerization; heart development; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; osteoclast differentiation; response to organic cyclic substance; regulation of apoptosis; hair cycle process; cell-cell adhesion; positive regulation of endothelial cell differentiation; organ development; ectoderm development; positive regulation of epithelial cell differentiation; cell adhesion; bone resorption; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of protein sumoylation; genitalia morphogenesis; patterning of blood vessels; endoderm formation; midgut development; negative regulation of transcription, DNA-dependent; cellular process; oocyte development; negative regulation of osteoclast differentiation; glial cell fate determination; endodermal cell fate commitment; cell-matrix adhesion; cell fate specification; embryonic hindlimb morphogenesis; response to estradiol stimulus; synaptic transmission; regulation of osteoclast differentiation; central nervous system vasculogenesis; vasculature development; regulation of transcription, DNA-dependent; regulation of cell differentiation; hemopoiesis; cell differentiation; fallopian tube development; skeletal development; layer formation in the cerebral cortex; hair follicle morphogenesis; thymus development; regulation of T cell proliferation; embryonic axis specification; synaptic vesicle transport; liver development; positive regulation of telomerase activity; negative regulation of oligodendrocyte differentiation; myoblast differentiation; response to cytokine stimulus