Western Blot (WB)
(Western blot analysis was performed by loading 25ug of various whole cell lysates probed with Rabbit anti RPAP3 at 1/1000 dilution. Arrow points to RPAP3 (molecular weight 76 kDa).)
Rabbit anti-Human RPAP3 Polyclonal Antibody | anti-RPAP3 antibody
RABBIT ANTI RPAP3
Reactivity
Human
Applications
Western Blot
Synonyms
RPAP3; Polyclonal Antibody; RABBIT ANTI RPAP3; RPAP3 antibody; anti-RPAP3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
RPAP3
Form/Format
Purified IgG, liquid. Phosphate buffered saline
Antiserum to RPAP3 was raised by repeated immunization of rabbits with highly purified antigen
Antiserum to RPAP3 was raised by repeated immunization of rabbits with highly purified antigen
Applicable Applications for anti-RPAP3 antibody
Western Blot (WB)
Application Notes
WB: 1/500-1/2000
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Species Cross-Reactivity
Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 17-241 of human RPAP3 (NP_001139547)
Preservative Stabilizers
0.02% Sodium Azide
50% Glycerol
50% Glycerol
Preparation and Storage
Store at -20 degree C
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Western Blot (WB)
(Western blot analysis was performed by loading 25ug of various whole cell lysates probed with Rabbit anti RPAP3 at 1/1000 dilution. Arrow points to RPAP3 (molecular weight 76 kDa).)
Western Blot (WB)
(Western blot analysis was performed by loading 25ug of various whole cell lysates probed with Rabbit anti RPAP3 at 1/1000 dilution. Arrow points to RPAP3 (molecular weight 76 kDa).)
Related Product Information for anti-RPAP3 antibody
Rabbit anti RPAP3 antibody recognizes RNA polymerase II-associated protein 3 (RPAP3), also known as HSpagh and Tah1.
It is a component of the R2TP/PFDL complex (Cloutier et al. 2009) and provides a flexible scaffold for allowing the abundant molecular chaperone HSP90 to interact with other proteins including RNA Pol II, TOR and SMG1, allowing the assembly of important cellular complexes (Martino et al. 2018). It has been suggested that RPAP3 can modulate UV-induced DNA damage (Ni et al. 2009). RPAP3 appears to bind to NEMO, inhibiting the NF-kappaB signaling pathway and enhancing cytotoxicity of the chemotherapeutic agent doxorubicin (Shimada et al. 2011).
It is a component of the R2TP/PFDL complex (Cloutier et al. 2009) and provides a flexible scaffold for allowing the abundant molecular chaperone HSP90 to interact with other proteins including RNA Pol II, TOR and SMG1, allowing the assembly of important cellular complexes (Martino et al. 2018). It has been suggested that RPAP3 can modulate UV-induced DNA damage (Ni et al. 2009). RPAP3 appears to bind to NEMO, inhibiting the NF-kappaB signaling pathway and enhancing cytotoxicity of the chemotherapeutic agent doxorubicin (Shimada et al. 2011).
Similar Products
Product Notes
The RPAP3 (Catalog #AAA225928) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI RPAP3 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPAP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1/500-1/2000 Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the RPAP3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPAP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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