Western Blot (WB)
(Western blot analysis was performed by loading 25ug of various whole cell lysates probed with Rabbit anti PCF11 at 1/1000 dilution. Arrow points to PCF11 (molecular weight 180 kDa).)
Rabbit anti-Human PCF11 Polyclonal Antibody | anti-PCF11 antibody
RABBIT ANTI PCF11
Reactivity
Human
Applications
Western Blot
Synonyms
PCF11; Polyclonal Antibody; RABBIT ANTI PCF11; PCF11 antibody; anti-PCF11 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
PCF11
Form/Format
Purified IgG, liquid. Phosphate buffered saline
Antiserum to PCF11 was raised by repeated immunization of rabbits with highly purified antigen.
Antiserum to PCF11 was raised by repeated immunization of rabbits with highly purified antigen.
Applicable Applications for anti-PCF11 antibody
Western Blot (WB)
Application Notes
WB: 1/500-1/2000
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1256-1555 of human PCF11 (NP_056969.2)
Preservative Stabilizers
0.02% Sodium Azide50% Glycerol
Preparation and Storage
Store at -20 degree C
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Western Blot (WB)
(Western blot analysis was performed by loading 25ug of various whole cell lysates probed with Rabbit anti PCF11 at 1/1000 dilution. Arrow points to PCF11 (molecular weight 180 kDa).)
Western Blot (WB)
(Western blot analysis was performed by loading 25ug of various whole cell lysates probed with Rabbit anti PCF11 at 1/1000 dilution. Arrow points to PCF11 (molecular weight 180 kDa).)
Related Product Information for anti-PCF11 antibody
Rabbit anti PCF11 antibody recognizes pre-mRNA cleavage complex 2 protein Pcf11, also known as KIAA0824.
PCF11 is important for 3' processing of pre-mRNA and transcription termination, links transcription with mRNA export, and can selectively attenuate expression of transcriptional regulators (Kamieniarz-Gdula et al. 2019). PCF11 is autoregulated, and removal of the regulatory mechanism leads to global activation of polyadenylation sites close to promotors (Wang et al. 2019).
PCF11 is important for 3' processing of pre-mRNA and transcription termination, links transcription with mRNA export, and can selectively attenuate expression of transcriptional regulators (Kamieniarz-Gdula et al. 2019). PCF11 is autoregulated, and removal of the regulatory mechanism leads to global activation of polyadenylation sites close to promotors (Wang et al. 2019).
Similar Products
Product Notes
The PCF11 (Catalog #AAA225931) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI PCF11 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCF11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1/500-1/2000 Flow Cytometry: Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the PCF11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCF11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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