Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ND5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Rabbit anti-Mouse, Rat ND5 Polyclonal Antibody | anti-ND5 antibody
ND5 Polyclonal Antibody
Gene Names
mt-Nd5; ND5
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Synonyms
ND5; Polyclonal Antibody; ND5 Polyclonal Antibody; mt-Nd5; MTND5; anti-ND5 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence Length
607
Applicable Applications for anti-ND5 antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 2000
Immunogen
A synthetic peptide of mouse ND5
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ND5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ND5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human tonsil using ND5 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human tonsil using ND5 antibody at dilution of 1:100 (40x lens).)
Product Categories/Family for anti-ND5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
67kDa
NCBI Official Full Name
NADH dehydrogenase subunit 5 (10 days neonate medulla oblongata cDNA, RIKEN full-length enriched library, clone:B830039K22 product:unclassifiable, full insert sequence)
NCBI Official Synonym Full Names
NADH dehydrogenase 5, mitochondrial
NCBI Official Symbol
mt-Nd5
NCBI Official Synonym Symbols
ND5
NCBI Protein Information
NADH dehydrogenase subunit 5
UniProt Protein Name
NADH-ubiquinone oxidoreductase chain 5
Protein Family
UniProt Gene Name
mt-Nd5
Uniprot Description
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
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Product Notes
The ND5 mt-nd5 (Catalog #AAA9132358) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ND5 Polyclonal Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ND5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 2000. Researchers should empirically determine the suitability of the ND5 mt-nd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ND5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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