Rabbit iNOS Polyclonal Antibody | anti-Nos2 antibody

Rabbit anti iNOS

Cat. Num. AAA225046

• Gene Names: Nos2; iNOS; Nos-2; Nos2a; i-NOS; NOS-II; MAC-NOS
• Reactivity: Mouse, Rat, Bovine
• Applications: Immunoprecipitation, Western Blot
• Purity: Purified whole serum by affinity chromatography on Protein A.
• Synonyms: iNOS; Polyclonal Antibody; Rabbit anti iNOS; iNOS Antibody; anti-Nos2 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat, Bovine
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified whole serum by affinity chromatography on Protein A.
• Form/Format: Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3), 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1144

• Applicable Applications for anti-Nos2 antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: WB: 1:1000
• Immunogen: Synthetic peptide corresponding to aa 1131-1144 of mouse iNOS
• Preperation: Antiserum to iNOS was raised by repeated immunisation of rabbits with highly purified antigen.
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred.; Storage in frost-free freezers is not recommended. This product should be stored undiluted.; Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.

Western Blot (WB)

(Western blot analysis of rat brain extract probed with Rabbit anti iNOS)

Related Product Information for anti-Nos2 antibody

Rabbit anti iNOS antibody recognizes inducible nitric oxide synthase also known as iNOS and NOS type II. This enzyme triggers the synthesis of the second messenger molecule, nitric oxide (NO), by immune cells such as macrophages in response to pathogen exposure. Inducible nitric oxide synthase also becomes activated in response to interleukin 1 beta and tumor necrosis factor alpha (Lowenstein and Padalko, 2004). In endothelia, the molecule is produced by eNOS (endothelial nitric oxide synthase) where it acts as a vasodilator and platelet inhibitor while in the brain NO is produced by nNOS (neuronal nitric oxide synthase) and functions as a neurotransmitter.

NCBI and Uniprot Product Information

• NCBI GI #: 266649
• NCBI GeneID: 18126
• UniProt Accession #: P29477; O70515; O70516; Q5SXT3; Q6P6A0; Q8R410
• Molecular Weight: 130,575 Da
• NCBI Official Full Name: Nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2, inducible
• NCBI Official Symbol: Nos2
• NCBI Official Synonym Symbols: iNOS; Nos-2; Nos2a; i-NOS; NOS-II; MAC-NOS
• NCBI Protein Information: nitric oxide synthase, inducible
• UniProt Protein Name: Nitric oxide synthase, inducible
• Protein Family: Inositol 3-kinase
• UniProt Gene Name: Nos2
• UniProt Synonym Gene Names: Inosl; Inducible NOS; iNOS; MAC-NOS

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7503239). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (PubMed:16373578). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 ().