Testing Data #1
(Human peripheral blood lymphocytes stained with Mouse anti Human CD16 (MBS224741) followed by Rabbit F(ab')2 anti Mouse IgG:FITC (MBS235160))
Mouse CD16 Monoclonal Antibody | anti-CD16 antibody
MOUSE ANTI HUMAN CD16
Gene Names
FCGR3B; CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
Applications
Flow Cytometry, Functional Assay, Functional Assay
Synonyms
CD16; Monoclonal Antibody; MOUSE ANTI HUMAN CD16; anti-CD16 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
KD1
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
233
Applicable Applications for anti-CD16 antibody
Flow cytometry (FC/FACS), Functional Assays (FN)*
Application Notes
Flow Cytometry: Use 100ul of the suggested working dilution to label 1x106 cells in 100ul.
Functional Assays: Application Note: This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
Functional Assays: Application Note: This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data #1
(Human peripheral blood lymphocytes stained with Mouse anti Human CD16 (MBS224741) followed by Rabbit F(ab')2 anti Mouse IgG:FITC (MBS235160))
Testing Data #1
(Human peripheral blood lymphocytes stained with Mouse anti Human CD16 (MBS224741) followed by Rabbit F(ab')2 anti Mouse IgG:FITC (MBS235160))
Testing Data #2
(Human peripheral blood lymphocytes stained with Mouse anti Human CD16: FITC(MBS224692))
Testing Data #2
(Human peripheral blood lymphocytes stained with Mouse anti Human CD16: FITC(MBS224692))
Related Product Information for anti-CD16 antibody
Mouse anti Human CD16 antibody, clone KD1 recognizes human CD16, a 50-65kD cell surface molecule, which is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages (Moretta et al. 1990), whereas CD16B is primarily expressed by granulocytes (Bonecchi et al. 1999). Clone KD1 recognizes both forms of CD16 and will therefore recognize all cell types expressing CD16. Mouse anti Human CD16, clone KD1 can be used to identify CD16 in a range of species including bovine (Boysen et al. 2010) and ovine (Elhmouzi-Younes et al. 2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,216 Da
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor III-B isoform 2
NCBI Official Synonym Full Names
Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
NCBI Official Symbol
FCGR3B
NCBI Official Synonym Symbols
CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III-B; Fc-gamma receptor IIIb (CD 16); fc-gamma RIII-beta; fc-gamma RIIIb; igG Fc receptor III-1
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III-B
Protein Family
UniProt Gene Name
FCGR3B
UniProt Synonym Gene Names
CD16B; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb
UniProt Entry Name
FCG3B_HUMAN
NCBI Description
The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1. [provided by RefSeq, Aug 2012]
Uniprot Description
Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Research Articles on CD16
Similar Products
Product Notes
The CD16 fcgr3b (Catalog #AAA224555) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD16 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Functional Assays (FN)*. Flow Cytometry: Use 100ul of the suggested working dilution to label 1x106 cells in 100ul. Functional Assays: Application Note: This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Researchers should empirically determine the suitability of the CD16 fcgr3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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