Rabbit anti-Human WTIP Polyclonal Antibody | anti-WTIP antibody

WTIP, CT (WTIP, Wilms tumor protein 1-interacting protein) (Biotin)

Cat. Num. AAA6363821

• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: WTIP; Polyclonal Antibody; CT (WTIP; Wilms tumor protein 1-interacting protein) (Biotin); Wilms tumor protein 1-interacting protein; anti-WTIP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-WTIP antibody: Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; ELISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: WTIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-503 amino acids from the C-terminal region of human WTIP.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(WTIP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WTIP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(WTIP Antibody (C-term) western blot analysis in NCI-H460,WiDr cell line lysates (35ug/lane).This demonstrates the WTIP antibody detected the WTIP protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of WTIP Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-WTIP antibody

WTIP may monitor slit diaphragm protein assembly, a specialized adherens junction characteristic of podocytes. In case of podocyte injury, it shuttles into the nucleus and acts as a transcription regulator that represses WT1-dependent transcription regulation, thereby translating changes in slit diaphragm structure into altered gene expression and a less differentiated phenotype (By similarity).

Product Categories/Family for anti-WTIP antibody

Antibodies; Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 122937269
• NCBI GeneID: 126374
• NCBI Accession #: NP_001073905.1
• NCBI GenBank Nucleotide #: NM_001080436.1
• UniProt Accession #: A6NIX2
• Molecular Weight: 45,124 Da
• NCBI Official Full Name: Wilms tumor protein 1-interacting protein
• NCBI Official Synonym Full Names: WT1 interacting protein
• NCBI Official Symbol: WTIP
• NCBI Protein Information: Wilms tumor protein 1-interacting protein
• UniProt Protein Name: Wilms tumor protein 1-interacting protein
• Protein Family: Wilms tumor protein 1-interacting protein
• UniProt Gene Name: WTIP
• UniProt Synonym Gene Names: WT1-interacting protein

Uniprot Description

Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates Hippo signaling pathway and antagonizes phosphorylation of YAP1. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. In podocytes, may play a role in the regulation of actin dynamics and/or foot process cytoarchitecture (). In the course of podocyte injury, shuttles into the nucleus and acts as a transcription regulator that represses WT1-dependent transcription regulation, thereby translating changes in slit diaphragm structure into altered gene expression and a less differentiated phenotype. Involved in the organization of the basal body (). Involved in cilia growth and positioning ().