Mouse CD28 Monoclonal Antibody | anti-CD28 antibody

MOUSE ANTI BOVINE CD28:Low Endotoxin

Cat. Num. AAA224432

• Applications: Flow Cytometry, Functional Assay, Functional Assay
• Synonyms: CD28; Monoclonal Antibody; MOUSE ANTI BOVINE CD28:Low Endotoxin; anti-CD28 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: CC220
• Form/Format: Low Endotoxin; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 219

• Applicable Applications for anti-CD28 antibody: Flow cytometry (FC/FACS), Functional Assays (FN)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ; Functional Assays: Minimum Dilution: 5
• Preparation: Purified IgG prepared by affinity chromatography on Protein A
• Buffer Solution: Phosphate buffered saline.
• Target Species: Bovine
• Preparation and Storage: Store at -20 degree C only (ship 4 degree C); Shelf Life: 18 months from date of despatch.

Related Product Information for anti-CD28 antibody

Mouse anti Bovine CD28, clone CC220, recognizes the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). Mouse anti Bovine CD28, clone CC220 has been shown to be able to stimulate bovine T lymphocytes in combination with anti-bovine CD3. For functional use, MyBioSource recommend the use of MBS224432. Clone CC220 has been published under the alternative designation F849CD10.

NCBI and Uniprot Product Information

• NCBI GI #: 30794304
• NCBI GeneID: 281050
• NCBI Accession #: NP_851347.1
• NCBI GenBank Nucleotide #: NM_181004.1
• UniProt Accession #: Q28071
• Molecular Weight: 25,143 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28
• NCBI Official Synonym Full Names: CD28 molecule<
• NCBI Official Symbol: CD28
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28; antigen CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: CD28
• UniProt Entry Name: CD28_BOVIN

Uniprot Description

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.