Rabbit anti-Human PSMB10 Polyclonal Antibody | anti-PSMB10 antibody

PSMB10, CT (PSMB10, LMP10, MECL1, Proteasome subunit beta type-10, Low molecular mass protein 10, Macropain subunit MECl-1, Multicatalytic endopeptidase complex subunit MECl-1, Proteasome MECl-1, Proteasome subunit beta-2i) (Azide free) (HRP)

Cat. Num. AAA6338176

• Gene Names: PSMB10; LMP10; MECL1; beta2i
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PSMB10; Polyclonal Antibody; CT (PSMB10; LMP10; MECL1; Proteasome subunit beta type-10; Low molecular mass protein 10; Macropain subunit MECl-1; Multicatalytic endopeptidase complex subunit MECl-1; Proteasome MECl-1; Proteasome subunit beta-2i) (Azide free) (HRP); Proteasome subunit beta-2i; anti-PSMB10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PSMB10.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PSMB10 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 214-242 in the C-terminal region of human PSMB10, UniProt Accession #P40306.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS6000330 in MCF-7 cell line lysates (35ug/lane). This demonstrates the PSMB10 antibody detected the PSMB10 protein (arrow).)

Related Product Information for anti-PSMB10 antibody

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.

Product Categories/Family for anti-PSMB10 antibody

Antibodies; Proteasomes

References

1. Bailey, S.D., et al. Diabetes Care (2010). 2. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). 3. Moschonas, A., et al. Mol. Cell. Biol. 28(20):6208-6222(2008). 4. Liu, Y., et al. DNA Seq. 18(4):257-264(2007). 5. Listovsky, T., et al. EMBO J. 23(7):1619-1626(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4506191
• NCBI GeneID: 5699
• NCBI Accession #: NP_002792.1
• NCBI GenBank Nucleotide #: NM_002801.3
• UniProt Accession #: P40306; Q5U098; B2R5J4
• Molecular Weight: 28,936 Da
• NCBI Official Full Name: proteasome subunit beta type-10
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, beta type, 10
• NCBI Official Symbol: PSMB10
• NCBI Official Synonym Symbols: LMP10; MECL1; beta2i
• NCBI Protein Information: proteasome subunit beta type-10; low molecular mass protein 10; macropain subunit MECl-1; multicatalytic endopeptidase complex subunit MECl-1; proteasome MECl-1; proteasome catalytic subunit 2i; proteasome subunit MECL1; proteasome subunit beta 7i; protea
• UniProt Protein Name: Proteasome subunit beta type-10
• Protein Family: Proteasome
• UniProt Gene Name: PSMB10
• UniProt Synonym Gene Names: LMP10; MECL1
• UniProt Entry Name: PSB10_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMB10: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Belongs to the peptidase T1B family.

Protein type: Protease; EC 3.4.25.1; Proteasome complex

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytosol

Molecular Function: threonine endopeptidase activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; cell morphogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; humoral immune response; regulation of apoptosis; T cell proliferation; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis