Mouse SIGLEC-10 Monoclonal Antibody | anti-SIGLEC-10 antibody

MOUSE ANTI HUMAN SIGLEC-10:FITC

Cat. Num. AAA224376

• Gene Names: SIGLEC10; SLG2; PRO940; SIGLEC-10
• Applications: Flow Cytometry, Functional Assay
• Synonyms: SIGLEC-10; Monoclonal Antibody; MOUSE ANTI HUMAN SIGLEC-10:FITC; anti-SIGLEC-10 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5G6
• Specificity: Clone 5G6 specifically recognises human Siglec-10 (Sialic acid-binding Ig-like lectin 10), a putative adhesion molecule and member of the Ig superfamily, expressed by monocytes, B cells, eosinophils, and at a higher level by a subpopulation of CD16+CD56- natural killer (NK) cells. ; ; Structurally, Siglec-10 is most similar to the CD33-related group of Siglecs, and preferentially binds to glycoconjugates containing alpha-2,3- or alpha-2,6-linked sialic acid. Studies have shown that Siglec-10 acts as a substrate for VAP-1 (Vascular adhesion protein-1), a glycoprotein expressed on endothelium during inflammation, which is involved in primary amine oxidation and leucocyte trafficking, see Kive, E. et al. for details. This interaction between Siglec-10 and VAP-1, implicates Siglec-10 in endothelial lymphocyte adhesion and in the modulation of the inflammatory microenvironment. ; ; Studies have shown that clone 5G6 does not cross-react with Siglecs 3, 5, 7, 8 and 9.
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 519

• Applicable Applications for anti-SIGLEC-10 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells. ; Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin; Preparation
• Immunogen: Recombinant human Siglec-10, fused with the Fc region of human IgG; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Human peripheral blood lymphocytes stained with Mouse anti Human siglec-10 (MBS224495) followed by Rabbit F(ab')2 anti Mouse IgG:FITC (MBS235160))

Testing Data #2

(Human peripheral blood lymphocytes stained with Mouse anti Human siglec-10:FITC (MBS224376))

Testing Data #3

(Human peripheral blood lymphocytes stained with A: Mouse anti Human Siglec-10 or B the corresponding isotype control; dual stained with Mouse anti Human CD19:APC (MBS210021))

Related Product Information for anti-SIGLEC-10 antibody

Clone 5G6 specifically recognises human Siglec-10 (Sialic acid-binding Ig-like lectin 10), a putative adhesion molecule and member of the Ig superfamily, expressed by monocytes, B cells, eosinophils, and at a higher level by a subpopulation of CD16+CD56- natural killer (NK) cells. Structurally, Siglec-10 is most similar to the CD33-related group of Siglecs, and preferentially binds to glycoconjugates containing alpha-2,3- or alpha-2,6-linked sialic acid. Studies have shown that Siglec-10 acts as a substrate for VAP-1 (Vascular adhesion protein-1), a glycoprotein expressed on endothelium during inflammation, which is involved in primary amine oxidation and leucocyte trafficking, see Kive, E. et al. for details. This interaction between Siglec-10 and VAP-1, implicates Siglec-10 in endothelial lymphocyte adhesion and in the modulation of the inflammatory microenvironment. Studies have shown that clone 5G6 does not cross-react with Siglecs 3, 5, 7, 8 and 9, see Munday J. et al. for details.

NCBI and Uniprot Product Information

• NCBI GI #: 283837876
• NCBI GeneID: 89790
• NCBI Accession #: NP_001164627.1
• NCBI GenBank Nucleotide #: NM_001171156.1
• UniProt Accession #: Q96LC7; Q3MIR5; Q6UXI8; Q96G54; Q96LC8; A8K1I5; A8K3C7; C9JJ33; C9JM10; F8W917
• Molecular Weight: 57,486 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 10 isoform 2
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin 10
• NCBI Official Symbol: SIGLEC10
• NCBI Official Synonym Symbols: SLG2; PRO940; SIGLEC-10
• NCBI Protein Information: sialic acid-binding Ig-like lectin 10; sialic acid binding Ig-like lectin 10 Ig-like lectin 7; siglec-like gene 2; siglec-like protein 2
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 10
• UniProt Gene Name: SIGLEC10
• UniProt Synonym Gene Names: SLG2; Siglec-10
• UniProt Entry Name: SIG10_HUMAN

NCBI Description

SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6 (MIM 604405).[supplied by OMIM, Jul 2002]

Uniprot Description

Siglec-10: a cell surface protein belonging to the immunoglobulin superfamily and the SIGLEC (sialic acid binding Ig-like lectin) family. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha2,3- or 2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Five alternatively spliced isoforms have been described.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: plasma membrane; integral to membrane; extracellular region

Molecular Function: carbohydrate binding

Biological Process: cell adhesion