Human Membrane Cofactor Protein ELISA Kit | MCP/CD46 elisa kit

Human Membrane Cofactor Protein ELISA Kit

Cat. Num. AAA762581

• Gene Names: CD46; MCP; TLX; AHUS2; MIC10; TRA2.10
• Reactivity: Human
• Synonyms: Membrane Cofactor Protein; Human Membrane Cofactor Protein ELISA Kit; MCP/CD46/Membrane Cofactor Protein/AHUS2/MIC10/CD46 molecule; complement regulatory protein/TLXcomplement regulatory protein/trophoblast leucocyte common antigen/trophoblast-lymphocyte cross-reactive antigen; MCP/CD46 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MBP. No significant cross-reactivity or interference between MBP and analogues was observed.
• Sequence Length: 369

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MBP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MBP were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MCP/CD46 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MBP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MBP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MBP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MBP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 262938
• NCBI GeneID: 4179
• Molecular Weight: 36,826 Da
• NCBI Official Full Name: membrane cofactor protein
• NCBI Official Synonym Full Names: CD46 molecule
• NCBI Official Symbol: CD46
• NCBI Official Synonym Symbols: MCP; TLX; AHUS2; MIC10; TRA2.10
• NCBI Protein Information: membrane cofactor protein
• UniProt Protein Name: Membrane cofactor protein
• Protein Family: Membrane cofactor protein
• UniProt Gene Name: CD46
• UniProt Synonym Gene Names: MCP; MIC10
• UniProt Entry Name: MCP_HUMAN

NCBI Description

The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]

Uniprot Description

CD46: Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement- mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T- cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations. 16 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: basolateral plasma membrane; cell surface; focal adhesion; Golgi apparatus; inner acrosomal membrane; integral to plasma membrane; plasma membrane

Molecular Function: cadherin binding; complement binding; endopeptidase activity; enzyme inhibitor activity; protein binding; receptor activity; viral receptor activity

Biological Process: adaptive immune response; complement activation, classical pathway; entry of virus into host cell; innate immune response; interleukin-10 production; negative regulation of catalytic activity; negative regulation of complement activation; positive regulation of interleukin-10 production; positive regulation of memory T cell differentiation; positive regulation of regulatory T cell differentiation; positive regulation of T cell proliferation; proteolysis; regulation of complement activation; regulation of Notch signaling pathway; single fertilization; T cell mediated immunity