Human Sialic acid-binding Ig-like lectin 10 ELISA Kit | SIGLEC10 elisa kit

Human Sialic acid-binding Ig-like lectin 10 ELISA Kit

Cat. Num. AAA765038

• Gene Names: SIGLEC10; SLG2; PRO940; SIGLEC-10
• Reactivity: Human
• Synonyms: Sialic acid-binding Ig-like lectin 10; Human Sialic acid-binding Ig-like lectin 10 ELISA Kit; SIGLEC10 (sialic acid-binding Ig-like lectin 10)/Siglec-10/SLG2/PRO940/sialic acid binding Ig-like lectin 10/SIGLEC-10/siglec-like gene 2/Siglec-like protein 2; SIGLEC10 elisa kit

• Reactivity: Human
• Sequence Length: 697

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SIGLEC10 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human SIGLEC10 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human SIGLEC10 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SIGLEC10 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human SIGLEC10 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 31377639
• NCBI GeneID: 89790
• NCBI Accession #: NP_149121.2
• NCBI GenBank Nucleotide #: NM_033130.4
• Molecular Weight: 57,486 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 10 isoform 1
• NCBI Official Synonym Full Names: sialic acid binding Ig like lectin 10
• NCBI Official Symbol: SIGLEC10
• NCBI Official Synonym Symbols: SLG2; PRO940; SIGLEC-10
• NCBI Protein Information: sialic acid-binding Ig-like lectin 10
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 10
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: SIGLEC10
• UniProt Synonym Gene Names: SLG2; Siglec-10
• UniProt Entry Name: SIG10_HUMAN

NCBI Description

SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6 (MIM 604405).[supplied by OMIM, Jul 2002]

Uniprot Description

Siglec-10: a cell surface protein belonging to the immunoglobulin superfamily and the SIGLEC (sialic acid binding Ig-like lectin) family. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha2,3- or 2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Five alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: extracellular region; integral to membrane; plasma membrane

Molecular Function: carbohydrate binding

Biological Process: cell adhesion