Goat RAB8A Polyclonal Antibody | anti-RAB8A antibody

GOAT ANTI HUMAN RAB8A (C-TERMINAL)

Cat. Num. AAA224069

• Gene Names: RAB8A; MEL; RAB8
• Reactivity: Bovine (Expected from Sequence), Dog (Expected from Sequence)
• Applications: ELISA, Western Blot
• Synonyms: RAB8A; Polyclonal Antibody; GOAT ANTI HUMAN RAB8A (C-TERMINAL); anti-RAB8A antibody

• Host: Goat
• Reactivity: Bovine (Expected from Sequence), Dog (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 207

• Applicable Applications for anti-RAB8A antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 25kDa in human cerebellum and kidney cell lysates; ELISA: Maximum Dilution: 1/32000; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.3ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin
• Buffer Solution: TRIS buffered saline
• Antiserum Preparation: Antiserum to human RAB8A (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-RAB8A antibody

Goat anti Human RAB8A antibody recognizes an epitope within the C-terminal (CT) region of the human RAS superfamily member Rab8a (Ras-related protein Rab-8A), otherwise known as oncogene c-mel, Rab8A is a small monomeric GTP/GDP-binding protein with a predicted molecular weight of ~24kDa which may play a role in vesicular trafficking and neurotransmitter release. The Rab family of proteins, originally described as Ras-like proteins in brain are the largest group of GTPases within the Ras superfamily with 61 protein members identified. Rab family members are involved in the regulation of intracellular vesicular transport and movement of proteins between endocytic and secretory organelles (Wennerberg et al. 2005). Rab8A is involved in vesicular traffic between the trans golgi network and the basoleteral aspect of the plasma membrane (Huber et al. 1993). Goat anti human Rab8a antibody has been successfully used for the detection of Rab8a in human intestinal epithelial cells using Western blotting (Vacca et al. 2014).

NCBI and Uniprot Product Information

• NCBI GI #: 16933567
• NCBI GeneID: 4218
• NCBI Accession #: NP_005361.2
• NCBI GenBank Nucleotide #: NM_005370.4
• UniProt Accession #: P61006; P24407; Q6FHV5; B4DEK7
• Molecular Weight: 23,564 Da
• NCBI Official Full Name: ras-related protein Rab-8A
• NCBI Official Synonym Full Names: RAB8A, member RAS oncogene family
• NCBI Official Symbol: RAB8A
• NCBI Official Synonym Symbols: MEL; RAB8
• NCBI Protein Information: ras-related protein Rab-8A; mel transforming oncogene (RAB8 homolog); mel transforming oncogene (derived from cell line NK14); mel transforming oncogene (derived from cell line NK14)- RAB8 homolog; oncogene c-mel; ras-associated protein RAB8
• UniProt Protein Name: Ras-related protein Rab-8A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB8A
• UniProt Synonym Gene Names: MEL; RAB8
• UniProt Entry Name: RAB8A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq, Jul 2008]

Uniprot Description

RAB8A: May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO/C1orf88. Interacts with BIRC6/bruce. Interacts with OCRL. Belongs to the small GTPase superfamily. Rab family.

Protein type: Motility/polarity/chemotaxis; G protein; Oncoprotein; G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: centrosome; cytoplasmic vesicle membrane; recycling endosome membrane; secretory granule membrane; nonmotile primary cilium; postsynaptic density; dendrite; phagocytic vesicle; cytosol; cilium; Golgi membrane; nucleoplasm; phagocytic vesicle membrane; synaptic vesicle; trans-Golgi network transport vesicle; cell soma; nucleolus; plasma membrane; nucleus

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; myosin V binding; protein kinase binding; Rab GTPase binding

Biological Process: regulation of long-term neuronal synaptic plasticity; metabolic process; organelle organization and biogenesis; protein secretion; regulation of protein transport; regulation of exocytosis; intracellular protein transport; synaptic vesicle exocytosis; axonogenesis; cellular response to insulin stimulus; Golgi vesicle fusion to target membrane; cilium biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; Rab protein signal transduction; vesicle docking during exocytosis