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Typical Testing Data/Standard Curve (for reference only)

Human Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) ELISA Kit | KAZALD1 elisa kit

Human Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) ELISA Kit

Gene Names
KAZALD1; BONO1; FKSG28; FKSG40; IGFBP-rP10
Reactivity
Human
Synonyms
Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1); Human Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) ELISA Kit; DFNA14; DFNA38; DFNA6; DIDMOAD; WFRS; WFS; Wolframin; KAZALD1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of KAZALD1. No significant cross-reactivity or interference between KAZALD1 and analogues was observed.
Sequence Length
166
Samples
Tissue homogenates, cell lysates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.115ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KAZALD1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KAZALD1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for KAZALD1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of KAZALD1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KAZALD1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to KAZALD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KAZALD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of KAZALD1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,525 Da
NCBI Official Full Name
kazal-type serine protease inhibitor domain-containing protein 1 isoform b
NCBI Official Synonym Full Names
Kazal type serine peptidase inhibitor domain 1
NCBI Official Symbol
KAZALD1
NCBI Official Synonym Symbols
BONO1; FKSG28; FKSG40; IGFBP-rP10
NCBI Protein Information
kazal-type serine protease inhibitor domain-containing protein 1
UniProt Protein Name
Kazal-type serine protease inhibitor domain-containing protein 1
UniProt Gene Name
KAZALD1

NCBI Description

This gene encodes a secreted member of the insulin growth factor-binding protein (IGFBP) superfamily. The protein contains an insulin growth factor-binding domain in its N-terminal region, a Kazal-type serine protease inhibitor and follistatin-like domain in its central region, and an immunoglobulin-like domain in its C-terminal region. Studies of the mouse ortholog suggest that this protein may function in bone development and bone regeneration. This gene is hypomethylated and over-expressed in high-grade glioma compared to low-grade glioma, and thus the hypomethylated gene may be associated with cell proliferation and the shorter survival of patients with high-grade glioma. It is also one of numerous genes found to be deleted in a novel 5.54 Mb interstitial deletion, which is associated with multiple congenital anomalies. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

Involved in the proliferation of osteoblasts during bone formation and bone regeneration. Promotes matrix assembly ().

Research Articles on KAZALD1

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Product Notes

The Human KAZALD1 kazald1 (Catalog #AAA453483) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA453483 ELISA Kit recognizes Human KAZALD1. It is sometimes possible for the material contained within the vial of "Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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