Rabbit anti-Human RAB8A Polyclonal Antibody | anti-RAB8A antibody

RAB8A Antibody

Cat. Num. AAA9406428

• Gene Names: RAB8A; MEL; RAB8
• Reactivity: Human
• Applications: Western Blot
• Purity: Antigen affinity purification.
• Synonyms: RAB8A; Polyclonal Antibody; RAB8A Antibody; MEL; RAB8; anti-RAB8A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total RAB8A protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 0.7 mg/ml (varies by lot)
• Sequence Length: 207

• Applicable Applications for anti-RAB8A antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500-1:2000
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to a region derived from internal residues of human Ras-related protein Rab-8A
• Target Name: RAB8A
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 10%SDS-PAGE Lysates (from left to right): Hela and HepG2 cell Amount of lysate: 30ug per lane Primary antibody: 1/800 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)

Related Product Information for anti-RAB8A antibody

The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1.

Product Categories/Family for anti-RAB8A antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 16933567
• NCBI GeneID: 4218
• NCBI Accession #: NP_005361.2
• NCBI GenBank Nucleotide #: NM_005370.4
• UniProt Accession #: P61006; P24407; Q6FHV5; B4DEK7
• Molecular Weight: 23,564 Da
• NCBI Official Full Name: ras-related protein Rab-8A
• NCBI Official Synonym Full Names: RAB8A, member RAS oncogene family
• NCBI Official Symbol: RAB8A
• NCBI Official Synonym Symbols: MEL; RAB8
• NCBI Protein Information: ras-related protein Rab-8A
• UniProt Protein Name: Ras-related protein Rab-8A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB8A
• UniProt Synonym Gene Names: MEL; RAB8
• UniProt Entry Name: RAB8A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq, Jul 2008]

Uniprot Description

RAB8A: May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO/C1orf88. Interacts with BIRC6/bruce. Interacts with OCRL. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein; G protein, monomeric, Rab; Motility/polarity/chemotaxis; Oncoprotein; G protein, monomeric

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: centrosome; cytoplasmic vesicle membrane; secretory granule membrane; nonmotile primary cilium; recycling endosome membrane; dendrite; postsynaptic density; phagocytic vesicle; cytosol; cilium; nucleoplasm; Golgi membrane; phagocytic vesicle membrane; trans-Golgi network transport vesicle; synaptic vesicle; cell soma; nucleolus; plasma membrane; nucleus

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; myosin V binding; protein kinase binding; Rab GTPase binding

Biological Process: regulation of long-term neuronal synaptic plasticity; metabolic process; organelle organization and biogenesis; protein secretion; regulation of exocytosis; regulation of protein transport; intracellular protein transport; synaptic vesicle exocytosis; Golgi vesicle fusion to target membrane; cellular response to insulin stimulus; axonogenesis; cilium biogenesis; mitotic cell cycle; Rab protein signal transduction; G2/M transition of mitotic cell cycle; vesicle docking during exocytosis