Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Glutamic acid decarboxylase autoantibody IgG (GAD-Ab-IgG) Standard Curve.)
Rat Glutamic Acid Decarboxylase Autoantibody IgG (GAD-Ab-IgG) ELISA Kit | GAD-Ab-IgG elisa kit
Rat Glutamic Acid Decarboxylase Autoantibody IgG (GAD-Ab-IgG) ELISA Kit
Reactivity
Rat
Synonyms
Glutamic Acid Decarboxylase Autoantibody IgG (GAD-Ab-IgG); Rat Glutamic Acid Decarboxylase Autoantibody IgG (GAD-Ab-IgG) ELISA Kit; GAD-Ab-IgG elisa kit
Reactivity
Rat
Specificity
Rat Glutamic acid decarboxylase autoantibody IgG (GAD-Ab-IgG) ELISA Kit has high sensitivity and excellent specificity for detection of Rat GAD-Ab-IgG. No significant cross-reactivity or interference between Rat GAD-Ab-IgG and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Glutamic acid decarboxylase autoantibody IgG (GAD-Ab-IgG) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Glutamic acid decarboxylase autoantibody IgG (GAD-Ab-IgG) Standard Curve.)
Related Product Information for GAD-Ab-IgG elisa kit
Description: This Rat Glutamic acid decarboxylase autoantibody IgG (GAD-Ab-IgG) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GAD-Ab-IgG. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated GAD-Ab-IgG and incubated. The competitive inhibition reaction is launched between with HRP labeled GAD-Ab-IgG and unlabeled GAD-Ab-IgG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of GAD-Ab-IgG in the sample. The color development is stopped and the intensity of the color is measured.
Background: Glutamate decarboxylase is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD uses PLP as a cofactor. In mammals, GAD exists in two isoforms encoded by two different genes - GAD1 and GAD2. These isoforms are GAD67 and GAD65 with molecular weights of 67 and 65 kDa, respectively. GAD1 and GAD2 are expressed in the brain where GABA is used as a neurotransmitter, GAD2 is also expressed in the pancreas. At least two more forms, GAD25 and GAD44 (embryonic; EGAD) are described in the developing brain. They are coded by the alternative transcripts of GAD1, I-80 and I-86: GAD25 is coded by both, GAD44 - only by I-80. GAD65 and GAD67 synthesize GABA at different locations in the cell, at different developmental times, and for functionally different purposes. GAD67 is spread evenly throughout the cell while GAD65 is localized to nerve terminals.
Background: Glutamate decarboxylase is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD uses PLP as a cofactor. In mammals, GAD exists in two isoforms encoded by two different genes - GAD1 and GAD2. These isoforms are GAD67 and GAD65 with molecular weights of 67 and 65 kDa, respectively. GAD1 and GAD2 are expressed in the brain where GABA is used as a neurotransmitter, GAD2 is also expressed in the pancreas. At least two more forms, GAD25 and GAD44 (embryonic; EGAD) are described in the developing brain. They are coded by the alternative transcripts of GAD1, I-80 and I-86: GAD25 is coded by both, GAD44 - only by I-80. GAD65 and GAD67 synthesize GABA at different locations in the cell, at different developmental times, and for functionally different purposes. GAD67 is spread evenly throughout the cell while GAD65 is localized to nerve terminals.
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Product Notes
The Rat GAD-Ab-IgG (Catalog #AAA9712637) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712637 ELISA Kit recognizes Rat GAD-Ab-IgG. It is sometimes possible for the material contained within the vial of "Glutamic Acid Decarboxylase Autoantibody IgG (GAD-Ab-IgG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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