Rabbit GLUTAMATE DECARBOXYLASE 1/2 Polyclonal Antibody | anti-Gad2 antibody

RABBIT ANTI GLUTAMATE DECARBOXYLASE 1/2

Cat. Num. AAA221561

• Gene Names: Gad2; gad65
• Reactivity: Cat, Human, Mouse
• Applications: Immunohistochemistry, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Synonyms: GLUTAMATE DECARBOXYLASE 1/2; Polyclonal Antibody; RABBIT ANTI GLUTAMATE DECARBOXYLASE 1/2; anti-Gad2 antibody

• Host: Rabbit
• Reactivity: Cat, Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This product recognises both molecular forms of glutamate decarboxylase, GAD1 (GAD67) and GAD2 (GAD65), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.
• Form/Format: Purified; Purified IgG - liquid
• Sequence Length: 585

• Applicable Applications for anti-Gad2 antibody: Immunohistology Frozen, ELISA (EIA), Immunofluorescence (IF), Immunohistology Paraffin, Western Blot (WB)
• Application Notes: Western Blot: A doublet at approximately 65/67 kDa can be expected; Immunohistology - Frozen: Minimum Dilution: 1/200; Maximum Dilution: 1/5000; Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/10000
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide sequence D-F-L-I-E-E-I-E-R-L-G-Q-D-L from the C terminus of rat GAD2; Histology Positive Control Tissue
• Antiserum Preparation: Antiserum to glutamic acid decarboxylase was raised by repeated immunisation of with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on protein A.
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-Gad2 antibody

This product recognises both molecular forms of glutamate decarboxylase, GAD1 (GAD67) and GAD2 (GAD65), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.

NCBI and Uniprot Product Information

• NCBI GI #: 6978871
• NCBI GeneID: 24380
• NCBI Accession #: NP_036695.1
• NCBI GenBank Nucleotide #: NM_012563.1
• UniProt Accession #: Q05683
• Molecular Weight: 65,402 Da
• NCBI Official Full Name: glutamate decarboxylase 2
• NCBI Official Synonym Full Names: glutamate decarboxylase 2
• NCBI Official Symbol: Gad2
• NCBI Official Synonym Symbols: gad65
• NCBI Protein Information: glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 2 (islet); glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; glutamic acid decarboxylase 65
• UniProt Protein Name: Glutamate decarboxylase 2
• Protein Family: Glutamate decarboxylase
• UniProt Gene Name: Gad2
• UniProt Synonym Gene Names: Gad65; GAD-65
• UniProt Entry Name: DCE2_RAT

NCBI Description

catalyzes synthesis of gamma-Aminobutyric acid (GABA), the most widely distributed inhibitory neurotransmitter [RGD, Feb 2006]

Uniprot Description

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - alanine, aspartate and glutamate; Lyase; EC 4.1.1.15; Other Amino Acids Metabolism - taurine and hypotaurine

Cellular Component: Golgi membrane; presynaptic membrane; synaptic vesicle membrane; perinuclear region of cytoplasm; axon; cytoplasm; synapse; cytoplasmic vesicle; cell junction; cytosol

Molecular Function: glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding

Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter biosynthetic process