SDS-PAGE
GAD65 / GAD2 recombinant protein
Recombinant Mouse GAD65/GAD2/GAD-2 Protein
Gene Names
Gad2; GAD65; Gad-2; GAD(65); 6330404F12Rik
Purity
> 90 % as determined by SDS-PAGE
Synonyms
GAD65 / GAD2; Recombinant Mouse GAD65/GAD2/GAD-2 Protein; 6330404F12Rik; GAD(65); Gad-2;GAD6; GAD65 / GAD2 recombinant protein
Host
Baculovirus-Insect Cells
Purity/Purification
> 90 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% glycerol, 3mM DTT, pH 8.0.
Concentration
0.48mg/mL. (varies by lot)
Sequence Positions
Met 1-Leu 585
Sequence Length
585
Species
Species
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method.
Preparation and Storage
Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.
SDS-PAGE
SDS-PAGE
Related Product Information for GAD65 / GAD2 recombinant protein
Background: Mouse glutamate decarboxylase 2, also known as glutamate decarboxylase 65 kDa isoform, 65 kDa glutamic acid decarboxylase, GAD2 and GAD65, is a member of the group II decarboxylase family. GAD2 is identified as a major autoantigen in insulin-dependent diabetes. GAD2 is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. GAD2 may also play a role in the stiff man syndrome. GAD2 is implicated in the formation of the gamma-aminobutyric acid (GABA), a neurotransmitter involved in the regulation of food intake. GABA is synthesized in brain by two isoforms of glutamic acid decarboxylase (Gad), GAD1 and GAD2. GAD1 provides most of the GABA in brain, but GAD2 can be rapidly activated in times of high GABA demand. Mice lacking GAD2 are viable whereas deletion of GAD1 is lethal. Deletion of GAD2 increased ethanol palatability and intake and slightly reduced the severity of ethanol-induced withdrawal.
Description: A DNA sequence encoding the mouse GAD2 (NP_032104.2) (Met 1-Leu 585) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
Description: A DNA sequence encoding the mouse GAD2 (NP_032104.2) (Met 1-Leu 585) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Mol_Mass: 65.4 kDa
AP_Mol_Mass: 58 kDa
AP_Mol_Mass: 58 kDa
NCBI Official Full Name
glutamate decarboxylase 2
NCBI Official Synonym Full Names
glutamic acid decarboxylase 2
NCBI Official Symbol
Gad2
NCBI Official Synonym Symbols
GAD65; Gad-2; GAD(65); 6330404F12Rik
NCBI Protein Information
glutamate decarboxylase 2
UniProt Protein Name
Glutamate decarboxylase 2
UniProt Gene Name
Gad2
UniProt Synonym Gene Names
Gad65; GAD-65
Uniprot Description
GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Carbohydrate Metabolism - butanoate; EC 4.1.1.15; Lyase; Other Amino Acids Metabolism - beta-alanine; Other Amino Acids Metabolism - taurine and hypotaurine
Chromosomal Location of Human Ortholog: 2 A3|2 15.15 cM
Cellular Component: axon; cytoplasm; cytoplasmic vesicle; cytosol; perinuclear region of cytoplasm; synapse; synaptic vesicle membrane
Molecular Function: glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding
Biological Process: glutamate decarboxylation to succinate
Research Articles on GAD65 / GAD2
Similar Products
Product Notes
The GAD65 / GAD2 gad2 (Catalog #AAA2546861) is a Recombinant Protein produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Met 1-Leu 585. It is sometimes possible for the material contained within the vial of "GAD65 / GAD2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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