Rabbit GAD2 Polyclonal Antibody | anti-GAD2 antibody

GAD2 Antibody

Cat. Num. AAA9402359

• Gene Names: GAD2; GAD65
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: GAD2; Polyclonal Antibody; GAD2 Antibody; GAD65; MGC161605; MGC161607; anti-GAD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total GAD2 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 585

• Applicable Applications for anti-GAD2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human GAD2.
• Target Name: GAD2
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of brian cell lysate using GAD2 antibody.)

Related Product Information for anti-GAD2 antibody

The enzyme glutamate decarboxylase (GAD) is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid (1). GAD1 (GAD67) and GAD2 (GAD65) are expressed in nervous and endocrine systems (2) and are thought to be involved in synaptic transmission (3) and insulin secretion (4), respectively. Autoantibodies against GAD2 may serve as markers for type I diabetes (5). Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2 (6).

Product Categories/Family for anti-GAD2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4503875
• NCBI GeneID: 2572
• NCBI Accession #: NP_000809.1
• NCBI GenBank Nucleotide #: NM_000818.2
• UniProt Accession #: Q05329; Q9UD87
• Molecular Weight: 65,411 Da
• NCBI Official Full Name: glutamate decarboxylase 2
• NCBI Official Synonym Full Names: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• NCBI Official Symbol: GAD2
• NCBI Official Synonym Symbols: GAD65
• NCBI Protein Information: glutamate decarboxylase 2
• UniProt Protein Name: Glutamate decarboxylase 2
• Protein Family: Glutamate decarboxylase
• UniProt Gene Name: GAD2
• UniProt Synonym Gene Names: GAD65; GAD-65
• UniProt Entry Name: DCE2_HUMAN

NCBI Description

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

Uniprot Description

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - taurine and hypotaurine; Lyase; EC 4.1.1.15

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: Golgi membrane; presynaptic membrane; synaptic vesicle membrane; perinuclear region of cytoplasm; axon; plasma membrane; cell junction; cytosol

Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding

Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process