GAD65 / GAD2 / GAD-2 recombinant protein

Human GAD65 / GAD2 / GAD-2 Protein (GST Tag)

Cat. Num. AAA2544983

• Gene Names: GAD2; GAD65
• Purity: > 90 % as determined by reducing SDS-PAGE.
• Synonyms: GAD65 / GAD2 / GAD-2; Human GAD65 / GAD2 / GAD-2 Protein (GST Tag); GAD65; GAD65 / GAD2 / GAD-2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile 200 Tirs, 150 mM NaCl, pH 8.0, 10 % glycerol, 1 mM GSH, 5%Trehalose, 5% Mannitol, 0.01% Tween-80
• Sequence Positions: Met 1-Leu 585

• Species: Human
• Tag: N-GST
• Endotoxin: < 1.0 EU per ?g as determined by the LAL method.
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water(80ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Ships on blue ice. Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

SDS-PAGE

Related Product Information for GAD65 / GAD2 / GAD-2 recombinant protein

Glutamate decarboxylase 2, also known as glutamate decarboxylase 65 kDa isoform, 65 kDa glutamic acid decarboxylase, GAD2 and GAD65, is a member of the group II decarboxylase family. GAD2 is identified as a major autoantigen in insulin-dependent diabetes. GAD2 is responsible for catalyzing the production of gamma-aminobutyric acid from Lglutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. GAD2 may also play a role in the stiff man syndrome. GAD2 is implicated in the formation of the gamma-aminobutyric acid (GABA), a neurotransmitter involved in the regulation of food intake. GABA is synthesized in brain by two isoforms of glutamic acid decarboxylase (Gad), GAD1 and GAD2. GAD1 provides most of the GABA in brain, but GAD2 can be rapidly activated in times of high GABA demand. Mice lacking GAD2 are viable whereas deletion of GAD1 is lethal. Deletion of GAD2 increased ethanol palatability and intake and slightly reduced the severity of ethanol-induced withdrawal.

NCBI and Uniprot Product Information

• NCBI GI #: 4503875
• NCBI GeneID: 2572
• NCBI Accession #: NP_000809.1
• NCBI GenBank Nucleotide #: NM_000818.2
• UniProt Accession #: Q05329; Q9UD87
• Molecular Weight: Mol_Mass: 92.6 kDa
• NCBI Official Full Name: glutamate decarboxylase 2
• NCBI Official Synonym Full Names: glutamate decarboxylase 2
• NCBI Official Symbol: GAD2
• NCBI Official Synonym Symbols: GAD65
• NCBI Protein Information: glutamate decarboxylase 2
• UniProt Protein Name: Glutamate decarboxylase 2
• UniProt Gene Name: GAD2
• UniProt Synonym Gene Names: GAD65; GAD-65

NCBI Description

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

Uniprot Description

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Carbohydrate Metabolism - butanoate; EC 4.1.1.15; Lyase; Other Amino Acids Metabolism - beta-alanine; Other Amino Acids Metabolism - taurine and hypotaurine

Chromosomal Location of Human Ortholog: 10p12.1

Cellular Component: plasma membrane

Molecular Function: glutamate decarboxylase activity; protein binding

Biological Process: neurotransmitter secretion; synaptic transmission