Rabbit HA-TAG Polyclonal Antibody | anti-HA-TAG antibody

RABBIT ANTI HA-TAG

Cat. Num. AAA221368

• Applications: ELISA, Immunohistochemistry, Western Blot
• Synonyms: HA-TAG; Polyclonal Antibody; RABBIT ANTI HA-TAG; anti-HA-TAG antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 571

• Applicable Applications for anti-HA-TAG antibody: ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: Keyhole limpet hemocyanin conjugated epitope tag peptide YPYDVPDYA (114-122) from haemagglutinin influenza. A cysteine residue was used to facilitate coupling at the C-terminal end.; Buffer Solution
• Antiserum Preparation: Antisera to Haemagglutinin epitope were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Viral
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-HA-TAG antibody

Rabbit anti HA-Tag antibody recognizes the HA (Haemagglutinin) epitope tag peptide sequence YPYDVPDYA, when fused to either the amino- or carboxyl- terminus of target proteins, including expression in many frequently used expression vectors. Rabbit anti HA-Tag antibody has been tested against both the immunogen and recombinant proteins containing the HA sequence in ELISA and Western blotting. In Western blotting of bacterial extracts Rabbit anti HA-Tag antibody has been shown not to cross-react with any endogenous proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 82016736
• UniProt Accession #: Q03909
• Molecular Weight: 64,105 Da
• NCBI Official Full Name: Hemagglutinin
• UniProt Protein Name: Hemagglutinin
• UniProt Gene Name: HA
• UniProt Entry Name: HEMA_I89A7

Uniprot Description

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore ().