Rabbit 5-HT7 RECEPTOR Polyclonal Antibody | anti-5-HT7 antibody

RABBIT ANTI HUMAN 5-HT7 RECEPTOR (C-TERMINAL)

Cat. Num. AAA222827

• Gene Names: HTR7; 5-HT7
• Reactivity: Mouse (Expected from Sequence)
• Applications: Immunohistochemistry
• Synonyms: 5-HT7 RECEPTOR; Polyclonal Antibody; RABBIT ANTI HUMAN 5-HT7 RECEPTOR (C-TERMINAL); anti-5-HT7 antibody

• Host: Rabbit
• Reactivity: Mouse (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects an epitope within the C-terminal of the serotonin (5-hydroxytryptamine, 5-HT) receptor 5-HT7, which belongs to the G-protein coupled receptor superfamily. Serotonin is a neurotransmitter and vasoactive amine which is thought to play a role in various cognitive and behavioural functions including pain, appetite, mood and sleep. ; ; Signalling through 5-HT7 has several effects, including regulation of the circadian rhythm and mediation of smooth-muscle relaxation in the vasculature and gastrointestinal tract. Signalling though 5-HT7 has also been shown to contribute to early T-cell activation. ; ; 5-HT7 has been implicated in depression, autism, and other neuropsychiatric disorders.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 445

• Applicable Applications for anti-5-HT7 antibody: Immunohistology Paraffin*
• Application Notes: Immunohistology - Paraffin: Maximum Dilution: 10ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Perservative Stabilisers: 0.1% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide C-terminal regoin of human 5-HT7 receptor, conjugated to Keyhole Limpet Hemocyanin (KLH).; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to 5-HT7 receptor (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human cerebellum with Rabbit anti Human 5-HT7 receptor (MBS222827))

Related Product Information for anti-5-HT7 antibody

MBS222827 detects an epitope within the C-terminal of the serotonin (5-hydroxytryptamine, 5-HT) receptor 5-HT7, which belongs to the G-protein coupled receptor superfamily. Serotonin is a neurotransmitter and vasoactive amine which is thought to play a role in various cognitive and behavioural functions including pain, appetite, mood and sleep. Signalling through 5-HT7 has several effects, including regulation of the circadian rhythm and mediation of smooth-muscle relaxation in the vasculature and gastrointestinal tract. Signalling though 5-HT7 has also been shown to contribute to early T-cell activation. 5-HT7 has been implicated in depression, autism, and other neuropsychiatric disorders.

NCBI and Uniprot Product Information

• NCBI GI #: 4504547
• NCBI GeneID: 3363
• NCBI Accession #: NP_000863.1
• NCBI GenBank Nucleotide #: NM_000872.4
• UniProt Accession #: P34969; P78336; P78372; P78516; Q5VX01; Q5VX02; Q5VX03; B5BUP6
• Molecular Weight: 47,984 Da
• NCBI Official Full Name: 5-hydroxytryptamine receptor 7 isoform a
• NCBI Official Synonym Full Names: 5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled
• NCBI Official Symbol: HTR7
• NCBI Official Synonym Symbols: 5-HT7
• NCBI Protein Information: 5-hydroxytryptamine receptor 7; 5-HT-7; 5-HT-X; 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled)
• UniProt Protein Name: 5-hydroxytryptamine receptor 7
• UniProt Gene Name: HTR7
• UniProt Synonym Gene Names: 5-HT-7; 5-HT7
• UniProt Entry Name: 5HT7R_HUMAN

NCBI Description

The neurotransmitter, serotonin, is thought to play a role in various cognitive and behavioral functions. The serotonin receptor encoded by this gene belongs to the superfamily of G protein-coupled receptors and the gene is a candidate locus for involvement in autistic disorder and other neuropsychiatric disorders. Three splice variants have been identified which encode proteins that differ in the length of their carboxy terminal ends. [provided by RefSeq, Jul 2008]

Uniprot Description

5-HT(7): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q21-q24

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: serotonin receptor activity

Biological Process: circadian rhythm; vasoconstriction; G-protein signaling, coupled to cyclic nucleotide second messenger; synaptic transmission; smooth muscle contraction; blood circulation; serotonin receptor signaling pathway