Western Blot (WB)
(GHRH Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GHRH antibody detected the GHRH protein (arrow).)
Rabbit anti-Human Growth Hormone Releasing Factor (HRP) Polyclonal Antibody | anti-GHRH antibody
Growth Hormone Releasing Factor (HRP)
Gene Names
GHRH; GRF; INN; GHRF; MGC119781
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
PurifiedPurified by ammonium sulfate precipitation.
Synonyms
Growth Hormone Releasing Factor (HRP); Polyclonal Antibody; Rabbit Anti-Growth Hormone Releasing Factor; NT (GHRF; GRF; Growth Hormone Releasing Hormone; GHRH; MGC119781; Sermorelin; Somatocrinin; Somatoliberin; Somatorelin) (HRP); anti-GHRH antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GHRH.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Applicable Applications for anti-GHRH antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
ELISA: 1:1000; Immunohistochemistry (Paraffin): 1:10-1:50; Western Blot: 1:50-1:100; Immunofluorescence: 1:10-1:50
Conjugation
HRP
Immunogen
Synthetic peptide corresponding to a portion of amino acids within aa 14-43 from the N-terminal region of human GHRH, conjugated to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(GHRH Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GHRH antibody detected the GHRH protein (arrow).)
Western Blot (WB)
(GHRH Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GHRH antibody detected the GHRH protein (arrow).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of GHRH Antibody (N-term) with 293 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of GHRH Antibody (N-term) with 293 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GHRH antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GHRH antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-GHRH antibody
GHRH belongs to the glucagon family and is a preproprotein that is produced in the hypothalamus. The preproprotein is cleaved to form a 44 aa factor, also called somatocrinin, that acts to stimulate growth hormone release from the pituitary. Variant receptors for somatocrinin have been found in several types of tumors, and antagonists of these receptors can inhibit the growth of the tumors. Defects in this protein are a cause of dwarfism, while hypersecretion of the encoded protein is a cause of gigantism.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
12,447 Da
NCBI Official Full Name
growth hormone releasing factor
NCBI Official Synonym Full Names
growth hormone releasing hormone
NCBI Official Symbol
GHRH
NCBI Official Synonym Symbols
GRF; INN; GHRF; MGC119781
NCBI Protein Information
somatoliberin; sermorelin; somatorelin; somatocrinin; OTTHUMP00000030915; OTTHUMP00000030916; growth hormone-releasing factor; growth hormone-releasing hormone
UniProt Protein Name
Somatoliberin
Protein Family
UniProt Gene Name
GHRH
UniProt Synonym Gene Names
GHRF
UniProt Entry Name
SLIB_HUMAN
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Product Notes
The GHRH ghrh (Catalog #AAA6120630) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Growth Hormone Releasing Factor (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Growth Hormone Releasing Factor (HRP) can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). ELISA: 1:1000; Immunohistochemistry (Paraffin): 1:10-1:50; Western Blot: 1:50-1:100; Immunofluorescence: 1:10-1:50. Researchers should empirically determine the suitability of the GHRH ghrh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Growth Hormone Releasing Factor (HRP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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