Testing Data #1
(Staining of formalin fixed paraffin processed human heart tissue with Rabbit anti Mouse Caspase-12 (NT) at 10 ug/ml. (MBS221650))
Rabbit anti-Human, Rat CASPASE-12 Polyclonal Antibody | anti-Casp12 antibody
RABBIT ANTI CASPASE-12 (aa2-17)
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Synonyms
CASPASE-12; Polyclonal Antibody; RABBIT ANTI CASPASE-12 (aa2-17); anti-Casp12 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1 mg/ml (varies by lot)
Sequence Length
419
Applicable Applications for anti-Casp12 antibody
Immunohistology Paraffin*, Western Blot (WB)
Application Notes
Immunohistology - Paraffin: Maximum Dilution: 10ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 0.5ug/ml; Maximum Dilution: 1ug/ml
Western Blotting: Minimum Dilution: 0.5ug/ml; Maximum Dilution: 1ug/ml
Perservative Stabilisers
0.02% Sodium Azide
Histology Positive Control Tissue
Heart tissue.
Buffer Solution
Buffer Solution
Antiserum Preparation
Antisera to mouse caspase 12 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data #1
(Staining of formalin fixed paraffin processed human heart tissue with Rabbit anti Mouse Caspase-12 (NT) at 10 ug/ml. (MBS221650))
Testing Data #1
(Staining of formalin fixed paraffin processed human heart tissue with Rabbit anti Mouse Caspase-12 (NT) at 10 ug/ml. (MBS221650))
Testing Data #2
(Western blot analysis of human (A) and mouse (B) spleen lysates probed with Rabbit anti caspase-12 (MBS221650))
Testing Data #2
(Western blot analysis of human (A) and mouse (B) spleen lysates probed with Rabbit anti caspase-12 (MBS221650))
Related Product Information for anti-Casp12 antibody
Rabbit anti caspase-12 (aa12-17) polyclonal antibody recognizes an epitope within the N-terminal region of murine caspase 12. Caspase 12 is a member of the interleukin-1 Beta converting enzyme (ICE) subfamily of caspases. Caspase 12 is ubiquitously expressed in murine tissue and is localised to the endoplasmic reticulum.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,854 Da
NCBI Official Full Name
caspase-12
NCBI Official Synonym Full Names
caspase 12
NCBI Official Symbol
Casp12
NCBI Protein Information
caspase-12; CASP-12
UniProt Protein Name
Caspase-12
Protein Family
UniProt Gene Name
Casp12
UniProt Synonym Gene Names
CASP-12
UniProt Entry Name
CASPC_MOUSE
Uniprot Description
CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; EC 3.4.22.-
Cellular Component: intracellular membrane-bound organelle; endoplasmic reticulum; intracellular; nucleus; cytosol
Molecular Function: peptidase activity; protein binding; protease binding; hydrolase activity; endopeptidase activity; cysteine-type peptidase activity
Biological Process: regulation of apoptosis; protein autoprocessing; positive regulation of apoptosis; apoptosis; unfolded protein response; positive regulation of caspase activity; proteolysis
Research Articles on Casp12
Similar Products
Product Notes
The Casp12 casp12 (Catalog #AAA221650) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI CASPASE-12 (aa2-17) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CASPASE-12 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Paraffin*, Western Blot (WB). Immunohistology - Paraffin: Maximum Dilution: 10ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Western Blotting: Minimum Dilution: 0.5ug/ml; Maximum Dilution: 1ug/ml. Researchers should empirically determine the suitability of the Casp12 casp12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CASPASE-12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
