Rabbit OGR1 Polyclonal Antibody | anti-OGR1 antibody

RABBIT ANTI HUMAN OGR1 (N-TERMINAL)

Cat. Num. AAA222838

• Gene Names: GPR68; OGR1; GPR12A
• Reactivity: Mouse (Expected from Sequence)
• Applications: Immunohistochemistry
• Synonyms: OGR1; Polyclonal Antibody; RABBIT ANTI HUMAN OGR1 (N-TERMINAL); anti-OGR1 antibody

• Host: Rabbit
• Reactivity: Mouse (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects ovarian cancer G protein-coupled receptor 1 (OGR1), a seven pass transmembrane protein initially cloned from an ovarian cancer cell line. OGR1 has a wide expression, and selectively binds both protons and bioactive lipids. ; ; OGR1 functions as a proton-sensing G protein-coupled receptor stimulating inositol phosphate production and Ca2+ mobilisation. It is also a receptor for sphingophosphorylcholine and related lysolipids, which antagonise the proton-induced actions mediated by OGR1. ; ; OGR1 is also expressed early during osteoclastogenesis and plays a role in osteoclast differentiation.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 365

• Applicable Applications for anti-OGR1 antibody: Immunohistology Paraffin*
• Application Notes: Immunohistology - Paraffin: Maximum Dilution: 20ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide: N-terminal domain of human OGR1, conjugated to Keyhole Limpet Hemocyanin (KLH).; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to human OGR1 (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human testis with Rabbit anti Human OGR1 (MBS222838))

Related Product Information for anti-OGR1 antibody

MBS222838 detects ovarian cancer G protein-coupled receptor 1 (OGR1), a seven pass transmembrane protein initially cloned from an ovarian cancer cell line. OGR1 has a wide expression, and selectively binds both protons and bioactive lipids. OGR1 functions as a proton-sensing G protein-coupled receptor stimulating inositol phosphate production and Ca2+ mobilisation. It is also a receptor for sphingophosphorylcholine and related lysolipids, which antagonise the proton-induced actions mediated by OGR1. OGR1 is also expressed early during osteoclastogenesis and plays a role in osteoclast differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 295054325
• NCBI GeneID: 8111
• NCBI Accession #: NP_001171147.1
• NCBI GenBank Nucleotide #: NM_001177676.1
• UniProt Accession #: Q15743; Q13334; Q4VBB4; Q6IX34
• Molecular Weight: 41,077 Da
• NCBI Official Full Name: ovarian cancer G-protein coupled receptor 1
• NCBI Official Synonym Full Names: G protein-coupled receptor 68
• NCBI Official Symbol: GPR68
• NCBI Official Synonym Symbols: OGR1; GPR12A
• NCBI Protein Information: ovarian cancer G-protein coupled receptor 1; G-protein coupled receptor 68; ovarian cancer G protein-coupled receptor, 1; sphingosylphosphorylcholine receptor
• UniProt Protein Name: Ovarian cancer G-protein coupled receptor 1
• Protein Family: Pentatricopeptide repeat-containing protein
• UniProt Gene Name: GPR68
• UniProt Synonym Gene Names: OGR1; OGR-1
• UniProt Entry Name: OGR1_HUMAN

Uniprot Description

GPR68: Proton-sensing receptor involved in pH homeostasis. May represents an osteoblastic pH sensor regulating cell-mediated responses to acidosis in bone. Mediates its action by association with G proteins that stimulates inositol phosphate (IP) production or Ca(2+) mobilization. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. Function also as a metastasis suppressor gene in prostate cancer. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Tumor suppressor; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 14q31

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of monocyte differentiation; inflammatory response