RAB32 recombinant protein

RAB32, 1-225aa, Human, His tag, E Coli

Cat. Num. AAA204486

• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: RAB32; 1-225aa; Human; His tag; E Coli; Ras-related protein Rab-32; RAB32 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 200mM NaCl, 2mM EDTA
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAGGGA GDPGLGAAAA PAPETREHLF KVLVIGELGV GKTSIIKRYV HQLFSQHYRA TIGVDFALKV LNWDSRTLVR LQLWDIAGQE RFGNMTRVYY KEAVGAFVVF DISRSSTFEA VLKWKSDLDS KVHLPNGSPI PAVLLANKCD QNKDSSQSPS QVDQFCKEHG FAGWFETSAK DNINIEEAAR FLVEKILVNH QSFPNEENDV DKIKLDQETL RAENKSQCC
• Sequence Length: 225

• Applicable Applications for RAB32 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RAB32 recombinant protein

RAB32 belongs to the small GTPase superfamily. RAB32 modulates ER calcium handling and disrupts the specific enrichment of calnexin on the MAM (mitochondria-associated membrane), while not affecting the ER distribution of protein-disulfide isomerase and mitofusin-2. Also, RAB32 determines the targeting of PKA (cAMP-dependent protein kinase) to mitochondrial and ER membranes and through its overexpression or inactivation increases the phosphorylation of Bad and of Drp1. Through a combination of its functions as a PKA-anchoring protein and a regulator of MAM properties, the activity and expression level of RAB32 determine the speed of apoptosis onset. Recombinant human RAB32 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for RAB32 recombinant protein

Signal Transduction

References

Bui M, et al. (2010) J Biol Chem. 285 (41):31590-602.; Alto NM, et al. (2002) J Cell Biol.158 (4):659-68.

NCBI and Uniprot Product Information

• NCBI GI #: 5803133
• NCBI GeneID: 10981
• NCBI Accession #: NP_006825
• NCBI GenBank Nucleotide #: NM_006834.4
• UniProt Accession #: Q13637
• Molecular Weight: 27.6kDa (249aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ras-related protein Rab-32
• NCBI Official Synonym Full Names: RAB32, member RAS oncogene family
• NCBI Official Symbol: RAB32
• NCBI Protein Information: ras-related protein Rab-32
• UniProt Protein Name: Ras-related protein Rab-32
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB32

NCBI Description

The protein encoded by this gene anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. The encoded protein also appears to be involved in autophagy and melanosome secretion. Variations in this gene may be linked to leprosy. [provided by RefSeq, Dec 2015]

Uniprot Description

Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission (PubMed:12186851). Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis (PubMed:21255211). Plays an important role in the control of melanin production and melanosome biogenesis (PubMed:23084991). In concert with RAB38, regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes ().