RAB1A recombinant protein

RAB1A, 1-205aa, Human, His tag, E Coli

Cat. Num. AAA203799

• Gene Names: RAB1A; RAB1; YPT1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: RAB1A; 1-205aa; Human; His tag; E Coli; Ras-related protein Rab-1A isoform 1; DKFZp564B163; RAB1; YPT1; RAB1A recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSSMNPEYDY LFKLLLIGDS GVGKSCLLLR FADDTYTESY ISTIGVDFKI RTIELDGKTI KLQIWDTAGQ ERFRTITSSY YRGAHGIIVV YDVTDQESFN NVKQWLQEID RYASENVNKL LVGNKCDLTT KKVVDYTTAK EFADSLGIPF LETSAKNATN VEQSFMTMAA EIKKRMGPGA TAGGAEKSNV KIQSTPVKQS GGGCC
• Sequence Length: 205

• Applicable Applications for RAB1A recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RAB1A recombinant protein

RAB1A, also known as ras-related protein Rab-1A, is a member of ras-related superfamily. Rab proteins play an important role for, either in endocytosis or in biosynthetic protein transport. Rab1a protein controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus. Recombinant human RAB1A protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for RAB1A recombinant protein

Signal Transduction

References

Zahraoui A, et al. (1989), J. Biol. Chem., 264: 12394-12401.; Chavrier P., et al. (1992). Gene. 112: 261-264.

NCBI and Uniprot Product Information

• NCBI GI #: 4758988
• NCBI GeneID: 5861
• NCBI Accession #: NP_004152
• NCBI GenBank Nucleotide #: NM_004161.4
• UniProt Accession #: P62820; P11476; Q6FIE7; Q96N61; Q9Y3T2
• Molecular Weight: 24.8 kDa (225aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ras-related protein Rab-1A isoform 1
• NCBI Official Synonym Full Names: RAB1A, member RAS oncogene family
• NCBI Official Symbol: RAB1A
• NCBI Official Synonym Symbols: RAB1; YPT1
• NCBI Protein Information: ras-related protein Rab-1A
• UniProt Protein Name: Ras-related protein Rab-1A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB1A
• UniProt Synonym Gene Names: RAB1

NCBI Description

This gene encodes a member of the Ras superfamily of GTPases. Members of the gene family cycle between inactive GDP-bound and active GTP-bound forms. This small GTPase controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus. Multiple alternatively spliced transcript variants have been identified for this gene which encode different protein isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport.