SDS-Page
MRAS recombinant protein
MRAS, 1-205aa, Human, His tag, E Coli
Gene Names
MRAS; M-RAs; RRAS3; R-RAS3
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
MRAS; 1-205aa; Human; His tag; E Coli; Ras-related protein M-Ras; M-RAs; R-RAS3; RRAS3; MRAS recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 5mM DTT, 40% glycerol, 200mM NaCl, 2mM EDTA
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MATSAVPSDN LPTYKLVVVG DGGVGKSALT IQFFQKIFVP DYDPTIEDSY LKHTEIDNQW AILDVLDTAG QEEFSAMREQ YMRTGDGFLI VYSVTDKASF EHVDRFHQLI LRVKDRESFP MILVANKVDL MHLRKITREQ GKEMATKHNI PYIETSAKDP PLNVDKAFHD LVRVIRQQIP EKSQKKKKKT KWRGDRATGT HKLQC
Sequence Length
208
Applicable Applications for MRAS recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for MRAS recombinant protein
MRAS (Ras-related protein M-Ras) belongs to the RAS superfamily. These families are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors. MRAS serve as an important signal transducer for novel upstream stimuli in controlling cell proliferation. Recombinant human MRAS protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for MRAS recombinant protein
References
Rodriguez-Viciana P., et al (2006) Mol. Cell 22:217-230; Quilliam LA, et al. (1999) J Biol Chem 274 (34): 23850-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25.7kDa (225aa), confirmed by MALDI-TOF
NCBI Official Full Name
ras-related protein M-Ras isoform 1
NCBI Official Synonym Full Names
muscle RAS oncogene homolog
NCBI Official Symbol
MRAS
NCBI Official Synonym Symbols
M-RAs; RRAS3; R-RAS3
NCBI Protein Information
ras-related protein M-Ras
UniProt Protein Name
Ras-related protein M-Ras
Protein Family
UniProt Gene Name
MRAS
UniProt Synonym Gene Names
RRAS3
NCBI Description
This gene encodes a member of the Ras family of small GTPases. These membrane-associated proteins function as signal transducers in multiple processes including cell growth and differentiation, and dysregulation of Ras signaling has been associated with many types of cancer. The encoded protein may play a role in the tumor necrosis factor-alpha and MAP kinase signaling pathways. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
Uniprot Description
May serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway.
Research Articles on MRAS
Similar Products
Product Notes
The MRAS mras (Catalog #AAA204770) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's MRAS can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the MRAS mras for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MATSAVPSDN LPTYKLVVVG DGGVGKSALT IQFFQKIFVP DYDPTIEDSY LKHTEIDNQW AILDVLDTAG QEEFSAMREQ YMRTGDGFLI VYSVTDKASF EHVDRFHQLI LRVKDRESFP MILVANKVDL MHLRKITREQ GKEMATKHNI PYIETSAKDP PLNVDKAFHD LVRVIRQQIP EKSQKKKKKT KWRGDRATGT HKLQC. It is sometimes possible for the material contained within the vial of "MRAS, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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