PPP1R8 recombinant protein

PPP1R8, 1-351aa, Human, His tag, E Coli

Cat. Num. AAA204250

• Gene Names: PPP1R8; ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PPP1R8; 1-351aa; Human; His tag; E Coli; Nuclear inhibitor of protein phosphatase 1; ARD-1; ARD1; NIPP-1; NIPP1; PRO2047; PPP1R8 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 2mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MAAAANSGSS LPLFDCPTWA GKPPPGLHLD VVKGDKLIEK LIIDEKKYYL FGRNPDLCDF TIDHQSCSRV HAALVYHKHL KRVFLIDLNS THGTFLGHIR LEPHKPQQIP IDSTVSFGAS TRAYTLREKP QTLPSAVKGD EKMGGEDDEL KGLLGLPEEE TELDNLTEFN TAHNKRISTL TIEEGNLDIQ RPKRKRKNSR VTFSEDDEII NPEDVDPSVG RFRNMVQTAV VPVKKKRVEG PGSLGLEESG SRRMQNFAFS GGLYGGLPPT HSEAGSQPHG IHGTALIGGL PMPYPNLAPD VDLTPVVPSA VNMNPAPNPA VYNPEAVNEP KKKKYAKEAW PGKKPTPSLL IVEHHHHHH
• Sequence Length: 351

• Applicable Applications for PPP1R8 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PPP1R8 recombinant protein

PPP1R8, also as known as NIPP-1, is the RNA-binding subunit of a major species of protein phosphatase-1 in the nucleus. This enzyme is inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. Recombinant human PPP1R8 protein, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PPP1R8 recombinant protein

Signal Transduction

References

Jin Q., et al. (1999) Biochem. J. 342:13-19; Beullens M., et al. (2000) Biochem. J. 352:651-658

NCBI and Uniprot Product Information

• NCBI GI #: 13699256
• NCBI GeneID: 5511
• NCBI Accession #: NP_054829
• NCBI GenBank Nucleotide #: NM_014110.4
• UniProt Accession #: Q12972; Q5TEJ2; Q5TEJ4; Q5TIF2; Q6PKF6; Q9UBH1; Q9UBZ0
• Molecular Weight: 39.5 kDa (359aa), confirmed by MALDI-TOF
• NCBI Official Full Name: nuclear inhibitor of protein phosphatase 1 isoform alpha
• NCBI Official Synonym Full Names: protein phosphatase 1 regulatory subunit 8
• NCBI Official Symbol: PPP1R8
• NCBI Official Synonym Symbols: ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
• NCBI Protein Information: nuclear inhibitor of protein phosphatase 1
• UniProt Protein Name: Nuclear inhibitor of protein phosphatase 1
• Protein Family: Nuclear inhibitor of protein phosphatase
• UniProt Gene Name: PPP1R8
• UniProt Synonym Gene Names: ARD1; NIPP1; NIPP-1

NCBI Description

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.