Rabbit anti-Human PPP1R8 Polyclonal Antibody | anti-PPP1R8 antibody

PPP1R8 Antibody

Cat. Num. AAA7044877

• Gene Names: PPP1R8; ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: PPP1R8; Polyclonal Antibody; PPP1R8 Antibody; Nuclear inhibitor of protein phosphatase 1; NIPP-1; Protein phosphatase 1 regulatory inhibitor subunit 8; Activator of RNA decay; ARD-1; ARD1; NIPP1; anti-PPP1R8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 127

• Applicable Applications for anti-PPP1R8 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Species: Human
• Immunogen: Recombinant human Nuclear inhibitor of protein phosphatase 1 protein (1-209AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-67702 / sc-67703 / sc-67704 / sc-67705
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: PPP1R8 antibody at 4.73 ug/mlLane 1: PC-3 whole cell lysateLane 2: HL60 whole cell lysateLane 3: HepG-2 whole cell lysateLane 4: Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 39,23,14 kDaObserved band size: 38 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7044877 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human pancreatic cancer using MBS7044877 at dilution 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of hepg2 using MBS7044877 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-PPP1R8 antibody

Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.

NCBI and Uniprot Product Information

• NCBI GI #: 4506015
• NCBI GeneID: 5511
• NCBI Accession #: NP_002704.1
• NCBI GenBank Nucleotide #: NM_002713.3
• UniProt Accession #: Q12972; Q5TEJ2; Q5TEJ4; Q5TIF2; Q6PKF6; Q9UBH1; Q9UBZ0
• Molecular Weight: 13,344 Da
• NCBI Official Full Name: nuclear inhibitor of protein phosphatase 1 isoform gamma
• NCBI Official Synonym Full Names: protein phosphatase 1 regulatory subunit 8
• NCBI Official Symbol: PPP1R8
• NCBI Official Synonym Symbols: ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
• NCBI Protein Information: nuclear inhibitor of protein phosphatase 1
• UniProt Protein Name: Nuclear inhibitor of protein phosphatase 1
• Protein Family: Nuclear inhibitor of protein phosphatase
• UniProt Gene Name: PPP1R8
• UniProt Synonym Gene Names: ARD1; NIPP1; NIPP-1
• UniProt Entry Name: PP1R8_HUMAN

NCBI Description

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]

Uniprot Description

NIPP-1: Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.-; Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis; Ribonuclease; Inhibitor

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: nuclear speck; nucleoplasm; nucleus

Molecular Function: protein binding; ribonuclease E activity; type 1 serine/threonine specific protein phosphatase inhibitor activity

Biological Process: RNA catabolic process