SecB recombinant protein

SecB, 1-155aa, E Coli, E Coli

Cat. Num. AAA203319

• Gene Names: secB; ECK3599; JW3584
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: SecB; 1-155aa; E Coli; Protein export chaperone; Protein export protein secB; SecB recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MSEQNNTEMT FQIQRIYTKD ISFEAPNAPH VFQKDWQPEV KLDLDTASSQ LADDVYEVVL RVTVTASLGE ETAFLCEVQQ GGIFSIAGIE GTQMAHCLGA YCPNILFPYA RECITSMVSR GTFPQLNLAP VNFDALFMNY LQQQAGEGTE EHQDA
• Sequence Length: 155

• Applicable Applications for SecB recombinant protein: SDS-PAGE
• Antigen Species: E Coli
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SecB recombinant protein

SecB, a remarkable chaperone involved in protein export, binds diverse ligands rapidly with high affinity and low specificity. It plays a crucial role during protein export via the general secretory pathway by modulating the partitioning of precursors between folding or aggregation and delivery to the membrane-bound translocation apparatus. SecB has the potential to participate in functions outside of export acting as a general nonspecific chaperone to provide buffering capacity of the nonnative state of proteins in the cytosolic pool. Recombinant E Coli SecB protein was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for SecB recombinant protein

Heat Shock Proteins

References

Driessen AJ., et al. (2008), Annu Rev Biochem. 77:643-67.; Bechtluft P., et al. (2007). Science. 318 (5855):1458-61.;

NCBI and Uniprot Product Information

• NCBI GI #: 16131480
• NCBI GeneID: 948123
• NCBI Accession #: NP_418066
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0AG86; P15040; Q2M7S3
• Molecular Weight: 17.2 kDa (155aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: protein export chaperone
• NCBI Official Symbol: secB
• NCBI Official Synonym Symbols: ECK3599; JW3584
• NCBI Protein Information: protein export chaperone
• UniProt Protein Name: Protein-export protein SecB
• Protein Family: SecB-like chaperone
• UniProt Gene Name: secB

NCBI Description

Penultimate Ser residue missing from purified protein could be a proteolytic artifact. [More information is available at EcoGene: EG10937]. SecB is a cytoplasmic protein involved in the Sec secretion pathway and is believed to function in conjunction with SecA/E/G/Y to chaperone proteins in their unfolded state to the inner membrane secretion complex. [More information is available at EcoCyc: EG10937].

Uniprot Description

One of the proteins required for the normal export of some preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. For 2 proteins (MBP, MalE and PhoA) the substrate is wrapped around the homotetramer, which prevents it from folding (PubMed:27501151). It also specifically binds to its receptor SecA. Its substrates include DegP, FhuA, FkpA, GBP, LamB, MalE (MBP), OmpA, OmpF, OmpT, OmpX, OppA, PhoE, TolB, TolC, YbgF, YcgK, YgiW and YncE (PubMed:16352602).